Back to results
US 5,393,661 AGranted
Three reagent gram staining method and kit
AssigneeDifco Laboratories
InventorsAmy T. Meszaros, Leon F. Strenkoski
Life of this patent
Estimating the term…
Abstract
A method for Gram staining a specimen containing bacteria includes the steps of staining Gram positive bacteria in the specimen, fixing the stain to the Gram positive bacteria, and simultaneously decolorizing and counter-staining Gram negative bacteria in the specimen.
Claims42
A method for Gram staining a specimen containing bacteria by:
- a) staining Gram positive bacteria in the specimen;
- b) fixing the stain to the Gram positive bacteria and;
- c) simultaneously decolorizing and counter-staining Gram negative bacteria in the specimen.
A kit for Gram staining containing reagents comprising:
- first reagent means for staining Gram positive bacteria;
- second reagent means for fixing the stain to the Gram positive bacteria;
- and
- third reagent means for simultaneously decolorizing and de-staining Gram negative bacteria.
1 more element
A Gram staining reagent for simultaneously decolorizing and counter-staining bacteria affixed to a slide consisting of a decolorizing solvent, a counter-stain, and a buffer.
Dependencies are inferred from the claim text.