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AssigneesToyo Boseki Kabushiki Kaisha, Ensuiko Sugar Refining Co., Ltd.
InventorsSumio Kitahata, Nobuhiro Kuwahara, Koki Fujita, Koji Hara, Keiichi Majima, Shin-ichi Teshima, Yuzo Hayashi
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A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.
A reagent for determining .alpha.-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR7## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosyl and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of said bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes.
A method for determining .alpha.-amylase activity in a sample, which comprises the steps of
Dependencies are inferred from the claim text.