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US 5,393,658 AGranted
Immunoassay method for the rapid identification of detergent treated antigens
AssigneeNew Horizons Diagnostics Corporation
InventorDuane A. Olsen
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AssigneeNew Horizons Diagnostics Corporation
InventorDuane A. Olsen
Estimating the term…
An assay is provided in which a target ligand is detected in a biological specimen. The method involves treating the target ligand with detergent and then binding the treated ligand to a particle by means of a capturing substance on the particle, thereby forming a complex. The complex is then applied to a porous membrane having a charge similar to that of a glass fiber filter in order to immobilize the complex in the porous membrane. The pore size of the membrane is substantially larger than the size of the complex such that the immobilization of the complex is substantially accomplished by means of the attraction of the complex to the charge of the porous membrane, and not as a function of the pore size of the membrane. Thereafter, the presence of the complex in the porous membrane is detected.
A method for detecting the presence of a target antigen in a biological specimen, comprising the steps of:
A kit for detecting the presence of a target antigen in a biological specimen, comprising:
Dependencies are inferred from the claim text.