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US 5,393,514 AGranted
Fluorescent pH indicators
AssigneeBecton, Dickinson and Company
InventorsJ. Bruce Pitner, Randal A. Hoke
Life of this patent
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Abstract
Fluorescent pH indicator compounds which exhibit an increase in fluorescence intensity with decreasing pH. The indicators are derivatives of rhodamine and sulforhodamine type dyes and are particularly useful for measuring pH in acidic environments such as carbon dioxide production by microorganisms and pH of certain acidic intracellular compartments.
Claims5
A method for determining the pH of a medium comprising:
- a) contacting the medium with a fluorescent pH indicator compound selected from the group consisting of compounds having the structure ##STR4## b) exposing the fluorescent compound to approximately 544 nm wavelength light to excite the fluorescent compound;
- c) measuring the intensity of fluorescence emitted by the excited fluorescent compound, and;
- d) determining the pH of the medium from the intensity of fluorescence.
Dependencies are inferred from the claim text.