Biosensor containing a biochemical substance immobilized on a layer of an olefinic-unsaturated, epoxyfunctional polyether

A biosensor with a selective detection system comprising a polymer and a biochemical substance wherein the detection system is produced in the following manner:

• applying an olefinic-unsaturated, epoxyfunctional polyether to a carrier material in the form of a layer,
• cross-linking the polyether by means of high-energy radiation to form an epoxyfunctional polymer matrix,
• treating the layer with an aqueous solution of the biochemical substance, whereby the biochemical substance is immobilized in the polymer matrix by reaction with epoxy groups, and
• stabilizing the layer by reaction of non-reacted epoxy groups with a compound containing an amino group, a carboxyl group or an amino group and a carboxyl group,
• wherein the polyether has the following structure:
• ##STR12## where the following applies:
• ##STR13## where R.sup.3 =--(CH.sub.2).sub.m --, with m=1 to 10
• R.sup.4 =H or CH.sub.3 ;
• R.sup.1 = ##STR14## with u=0 to 150, --(CH.sub.2).sub.o --, with o=0 to 18, or
• CH.sub.2 --O--R.sup.5 --O--CH.sub.2 --,
• where
• R.sup.5 =--(CH.sub.2).sub.p --, ##STR15## with p=2 to 20, q=2 to 4, r=1 to 50,
• s=0 to 50, t=0 to 25,
• Ar= ##STR16## R.sup.2 =--(CH.sub.2 --CH.dbd.CH--CH.sub.2).sub.n --, --R.sup.6 --, --R.sup.6 --O--CO--R.sup.7 --CO--O--R.sup.6 -- or ##STR17## where n=1 to 50, u=0 to 150,
• R.sup.6 has the same meaning as R.sup.5,
• except that ##STR18## are excluded, and R.sup.7 has the following meaning:
• ##STR19## with q=2 to 4, s=0 to 50, t=0 to 25,
• v=0 to 20, and
• Ar has the meaning indicated above.