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AssigneeRhein Biotech
InventorsCornelius P. Hollenberg, Zbigniew Janowicz
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The invention relates to DNA-molecules comprising DNA-sequences encoding control regions and the structural gene for a protein having formate dehydrogenase (FMDH) activity. Said DNA-molecules may be combined with DNA-sequences encoding foreign genes so as to bring these genes under the stringent control of the regulation of the FMDH regulatory sequences and/or may be combined to DNA-sequences coding for secretory signals. The invention further relates to recombinant vectors containing said DNA-molecules and micro-organisms containing said vectors or DNA-molecules. Furthermore, the invention relates to a process for producing a useful substance by producing this substance by culturing said micro-organisms and recovering the substance.
A process for producing a protein or polypeptide, comprising culturing a methylotrophic yeast containing a recombinant vector that contains an isolated DNA molecule comprising a 5' control region obtainable from a gene from a methylotrophic species of the genus Hansenula, said gene coding for a protein having formate dehydrogenase (FMDH) activity, derepressing said 5' control region by growth on glycerol as a carbon source, cultivating the methylotrophic yeast, and recovering the protein or polypeptide.
An isolated DNA molecule comprising a 5' control region which is derepressed when grown on glycerol as the only carbon source and which is induced when methanol is added, said 5' control region obtainable from a gene from a methylotrophic species of the genus Hansenula, said gene coding for a protein having formate dehydrogenase (FMDH) activity.
An isolated DNA molecule which comprises a DNA molecule obtainable from a gene from a methylotrophic species of Hansenula, said gene coding for a protein having formate dehydrogenase (FMDH) activity.
Dependencies are inferred from the claim text.