Sulfonamide bonded hydrophilic coatings
AssigneePerSeptive Biosystems, Inc.
InventorsNoubar B. Afeyan, Hossein K. Hodjat, Laszlo Varady
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Abstract
A method of derivatizing a chromatography matrix having a hydrophobic surface involves reacting the hydrophobic surface with a halosulfonating agent to produce a significant amount of halosulfone groups covalently bonded onto the surface. Sulfonamide bonds then are formed between these halosulfone groups and a group of amine functions on a polyaminated polymer to produce a pellicular anion exchange layer covalently bonded to the surface. The resulting layer is stable in the absence of cross-links between the molecules of the polyaminated polymer.
Claims13
A method of preparing a chromatography matrix having an anion exchange layer covalently bonded to a surface of a hydrophobic organic polymer comprising the steps of:
- a. reacting said hydrophobic polymer at a temperature above 4.degree. C. with a halosulfonating agent selected from the group consisting of chlorosulfonic acid and fluorosulfonic acid in a concentration of at least 10 mmol per gram of said polymer, thereby selectively forming halosulfone groups covalently bonded onto said polymer and minimizing formation of sulfonate groups;
- b. washing the halosulfonated polymer formed in step (a) with water or an organic solvent;
- and
- c. reacting said halosulfone groups with a polyaminated polymer having plural amine groups per molecule of said polymer to form sulfonamide bonds between said halosulfonated polymer and said plural amine groups of said polyaminated polymer thereby producing a non-cross-linked polymeric anion exchange layer covalently bonded to said hydrophobic polymer, said layer being stable in the absence of cross-links between the molecules of said polyaminated polymer.
A chromatography material comprising a hydrophobic, aromatic, organic polymeric substrate having on the surface thereof, a non-crosslinked hydrophilic polymeric layer, said layer comprising a polyaminated polymer having plural amine groups per molecule of said polymer covalently attached to said substrate by sulfonamide bonds, said layer being stable in the absence of cross-links between the molecules of said polyaminated polymer.
Dependencies are inferred from the claim text.