PO438, a new calcium-regulated promoter
AssigneeLaboratorios Serono S.A.
InventorsJose D. Ortega, Jose A. Gil, Tomas V. Garcia, Juan F. Martin
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Abstract
There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptides, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.
Claims10
A DNA fragment comprising the calcium-regulated promoter contained in FIG. 4(SEQ ID NO:1) or a fragment thereof having calcium regulated promoter activity.
A DNA fragment comprising the calcium-regulated promoter contained in SEQ ID NO:1 which is oriented in a direction opposite to the reading frame of ORF438.
Dependencies are inferred from the claim text.