Method for producing a mammalian G protein coupled glutamate receptor
AssigneesZymoGenetics, Inc., The Board of Regents of the University of Washington
InventorsEileen R. Mulvihill, Frederick S. Hagen, Khaled M. Houamed, Wolfhard Almers
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Abstract
Mammalian G protein coupled glutamate receptors are identified, isolated and purified. The receptors have been cloned, sequenced and expressed by recombinant means. The receptors and antibodies thereby may be used to identify agonists and antagonists of G protein coupled glutamate receptor mediated neuronal excitation, as well as in methods of diagnosis.
Claims17
An isolated and purified polynucleotide molecule which hybridizes at high stringency to an oligonucleotide of 25 or more contiguous nucleotides of SEQ ID NO:
- 1 and which codes for:
- a) a mammalian G protein coupled glutamate receptor;
- or
- b) a ligand binding domain or a transmembrane domain of a mammalian G protein coupled glutamate receptor.
A probe which comprises an oligonucleotide of 25 or more contiguous nucleotides of SEQ ID NO:
- 1 capable of specifically hybridizing with a gene which encodes a mammalian G protein coupled glutamate receptor, or allelic and species variants thereof.
A DNA construct comprising the following operably linked elements:
- a transcriptional promoter;
- a DNA sequence which hybridizes at high stringency to an oligonucleotide of 25 or more contiguous nucleotides of SEQ ID NO:
- 1 and which encodes (a) a mammalian G protein coupled glutamate receptor;
- or (b) a ligand binding domain or a transmembrane domain of a mammalian G protein coupled glutamate receptor;
- and
- a transcriptional terminator.
A cultured eukaryotic cell transformed or transfected with a DNA construct which comprises the following operably linked elements:
- a transcriptional promoter;
- a DNA sequence which hybridizes at high stringency to an oligonucleotide of 25 or more contiguous nucleotides of SEQ ID NO:
- 1 and which encodes (a) a mammalian G protein coupled glutamate receptor;
- or (b) a ligand binding domain or a transmembrane domain of a mammalian G protein coupled glutamate receptor;
- and
- a transcriptional terminator.
A method for producing a mammalian G protein coupled glutamate receptor having the amino acid sequence of SEQ ID NO:
- 2, which comprises:
- growing eukaryotic cells transformed or transfected with a DNA construct which comprises a DNA sequence of SEQ ID NO:
- 1 coding for the expression of the G protein coupled glutamate receptor, and
- isolating the receptor from the cells.
Dependencies are inferred from the claim text.