Synthetic insecticidal crystal protein gene
AssigneeMycogen Plant Science, Inc.
InventorsMichael J. Adang, Thomas A. Rocheleau, Donald J. Merlo, Elizabeth E. Murray
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Abstract
Synthetic Bacillus thuringiensis toxin genes designed to be expressed in plants at a level higher than naturally-occurring Bt genes are provided. These genes utilize codons preferred in highly expressed monocot or dicot proteins.
Claims14
A method of designing a synthetic Bacillus thuringiensis gene to be more highly expressed in plants, comprising the steps of:
- analyzing the coding sequence of a gene derived from a Bacillus thuringiensis which encodes an insecticidal protein toxin, and
- modifying a portion of said coding sequence to yield a modified sequence which contains a greater number of codons preferred by the intended plant host than did said coding sequence.
A method of designing a synthetic Bacillus thuringiensis gene to be more highly expressed in plants, comprising the steps of:
- analyzing the coding sequence of a gene derived from a Bacillus thuringiensis which encodes an insecticidal protein toxin, and modifying a portion of said coding sequence to yield a modified sequence which has a frequency of codon usage which more closely resembles the frequency of codon usage of the plant in which it is to be expressed.
A synthetic gene comprising the DNA sequence presented in FIG. 1, spanning nucleotides 1 through 1793.
A synthetic gene comprising the DNA sequence presented in FIG. 1, spanning nucleotides 1 through 1833.
Dependencies are inferred from the claim text.