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US 5,380,826 AGranted
Supercritical fluid disruption of and extraction from microbial cells
AssigneeAphios Corporation
InventorsTrevor P. Castor, Glenn T. Hong
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AssigneeAphios Corporation
InventorsTrevor P. Castor, Glenn T. Hong
Estimating the term…
The invention involves the supercritical or near-critical fluid disruption of microbial cells and extraction of intracellular components. First, a solvent that is a gas at ambient conditions and that has a critical temperature of between 0.degree. and 100.degree. C. is selected. This solvent is brought to near-critical pressure or higher and to near-critical temperature. The solvent then is combined with a slurry of cells to saturate the cells with the solvent under the prescribed conditions. Next, the pressure is released to cause a pressure drop which results in partial disruption of the cell membrane and release of solvent and other materials from the cell. Novel apparatus and associated methods are provided for carrying out the foregoing process continuously.
A method for collecting proteins or nucleic acids from intact prokaryotes comprising,
A method for collecting proteins or nucleic acids from intact eukaryotes comprising,
A method for selectively collecting protein from intact procaryotic cells comprising, combining a solvent with the procaryotic cells to form a combination, said solvent having a critical temperature and a critical pressure,
A method for collecting proteins or nucleic acids from intact prokaryotes comprising the steps:
A method for collecting proteins or nucleic acids from intact eukaryotes comprising, the steps:
Dependencies are inferred from the claim text.