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US 5,378,616 AGranted
Mutant Escherichia coli capable of enhanced L-glutamic acid production by fermentation
AssigneeAjinomoto Co., Inc.
InventorsNobuharu Tujimoto, Yoshimi Kikuchi, Osamu Kurahashi, Yoshiko Kawahara
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Abstract
The present invention provides a mutant and a process for producing L-glutamic acid by fermentation using a microorganism belonging to the genus Escherichia. In the present process, L-glutamic acid is produced and accumulated in a culture medium by (A) culturing an Escherichia mutant which is deficient or low in .alpha.-ketoglutaric acid dehydrogenase activity, has low L-glutamic acid decomposing ability, and is capable of producing L-glutamic acid.
Claims2
A process for producing L-glutamic acid by fermentation which comprises:
- (A) culturing in a culture medium, a mutant which is derived from Escherichia coli K-12 strain, wherein said mutant (i) is deficient or low in .alpha.-KGDH activity, (ii) is low in glutamic acid decomposition activity, (iii) is able to produce L-glutamic acid and (iv) expresses a malate synthase, an isocitrate lyase and an isocitrate dehydrogenase kinase/phosphatase;
- and
- (B) isolating L-glutamic acid from the culture medium.
Dependencies are inferred from the claim text.