Culture medium for production of recombinant protein
AssigneesJuridical Foundation The Chemo-Sero-Therapeutic Research Institute, Teijin Limited
InventorsKazuyuki Nakashima, Izumi Mimaki, Takayoshi Hamamoto, Kenichi Masuda
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Abstract
A culture medium without or with a low level of protein for culturing a transformed animal cell capable of continuously producing a protein prepared by using a genetic engineering technique, which contains a nonionic surfactant and cyclodextrin, and a method for producing a protein which comprises culturing a transformed animal cell capable of producing the desired protein prepared by using a genetic engineering technique in the culture medium.
Claims11
A method for producing coagulation Factor VIII which comprises culturing a Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant and a cyclodextrin and collecting the secreted coagulation Factor VIII from the culture medium, wherein the nonionic surfactant is selected from the group consisting of a pluronic surfactant and a sorbitan surfactant.
A method for producing coagulation Factor VIII which comprises culturing a Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant selected from the group consisting of a PLURONIC surfactant and a sorbitan surfactant, and a cyclodextrin and collecting the secreted coagulation Factor VIII from the culture medium, wherein the cyclodextrin is selected from the group consisting of unmodified .alpha.-cyclodextrin and methylated .alpha.-cyclodextrin.
A method for producing coagulation Factor VIII which comprises culturing transformed Chinese Hamster Ovary cell which produces said coagulation Factor VIII, in a culture medium comprising a nonionic surfactant selected from the group consisting of a PLVRONIC surfactant and a sorbitan surfactant, butyric acid or a salt thereof in a concentration of from 0.1 to 5.0 mM, a lithium salt in a concentration of from 2.0 to 50 mM, and a cyclodextrin, and collecting the secreted coagulation Factor VIII from the culture medium.
A culture medium, optionally containing a low level of protein, for culturing a transformed Chinese Hamster Ovary Cell which produces coagulation Factor VIII, which comprises a nonionic surfactant selected from the group consisting of a PLURONIC surfactant and a sorbitan surfactant and a cyclodextrin in admixture with a conventional culture medium.
The culture medium of claim. 6 wherein the nonionic surfactant is a PLURONIC surfactant selected from the group consisting of PLURONIC F-61, PLURONIC F-68, PLURONIC F-71 and PLURONIC F-108 or a sorbitan surfactant selected from the group consisting of polyoxyethylene sorbitan monolaurate and polyoxyethylene sorbitan monooleate.
Dependencies are inferred from the claim text.