Spectroscopic imaging device employing imaging quality spectral filters

A spectroscopic imaging device employing imaging quality spectral filters suitable for use in Raman emission microscopy comprising:

• a high intensity monochromatic light source;
• means for directing said monochromatic light toward a subject to be analyzed;
• collimation means for directing light emitted from each of a plurality of spatial locations within said subject in response to said monochromatic light source impinging upon said subject at an acousto-optic tunable filter, the acousto-optic tunable filter being of the type which is optically tunable by applying an input signal of a selected frequency to the filter;
• means operatively connected to the acousto-optic tunable filter for applying said input signal to the acousto-optic tunable filter thereby selecting a desired wavelength of said emitted light to be filtered by the acousto-optic tunable filter and passed through the acousto-optic tunable filter while maintaining image fidelity of the subject;
• and
• means for directing said filtered light at a focal plane array detector comprising a two-dimensional array of charge coupled devices, including collimation means and one or more Raman filters, wherein said charge coupled devices of said focal plane array detector measure the intensity of emitted light from each of said plurality of spatial locations of the subject.

A spectroscopic imaging device employing imaging quality spectral filters suitable for use in Fluorescence emission microscopy comprising:

• a substantially monochromatic light source;
• means for directing said monochromatic light toward a subject to be analyzed;
• collimation means for directing fluorescent light emitted from each of a plurality of spatial locations within said subject in response to said monochromatic light source impinging upon said subject at an acousto-optic tunable filter, the acousto-optic tunable filter being of the type which is optically tunable by applying an input signal of a selected frequency to the filter;
• means operatively connected to the acousto-optic tunable filter for applying said input signal to the acousto-optic tunable filter thereby selecting a desired wavelength of said fluorescent light to be filtered by the acousto-optic tunable filter and passed through the acousto-optic tunable filter while maintaining image fidelity of the subject;
• and
• means for collimating and directing said filtered light at a focal plane array detector comprising a two-dimensional array of charge coupled devices, wherein said charge coupled devices of said focal plane array detector measures the intensity of the filtered light from each of said plurality of spatial locations of the subject.

A spectroscopic imaging device employing imaging quality spectral filters suitable for use in Raman emission microscopy comprising:

• a high intensity monochromatic light source;
• means for directing said monochromatic light toward a subject to be analyzed;
• collimation means for directing light emitted from each of a plurality of spatial locations within said subject in response to said monochromatic light source impinging upon said subject at a step-scan interferometer, the step-scan interferometer being of the type which has at least one movable mirror which can be incrementally retarded to produce a multiplexed spectral output of the light passing through the interferometer;
• means operatively connected to the interferometer for selecting known retardations of said movable mirror of said interferometer, wherein said step-scan interferometer maintains the image fidelity of the subject as said emitted light passes through the interferometer;
• and
• means for collimating and directing said emitted light passing through the interferometer at a focal plane array detector comprising a two-dimensional array of charge coupled devices, wherein said charge coupled devices of said focal plane array detector measures the intensity of emitted light from each of said plurality of spatial locations of the subject at each of a plurality of said known retardations of the movable mirror.

A spectroscopic imaging device employing imaging quality spectral filters suitable for use in Fluorescence emission microscopy comprising:

• a substantially monochromatic light source;
• means for directing said monochromatic light toward a subject to be analyzed;
• collimation means for directing fluorescent light emitted from each of a plurality of spatial locations within said subject in response to said monochromatic light source impinging upon said subject at a step-scan interferometer, the step-scan interferometer being of the type which has at least one movable mirror which can be incrementally retarded to produce a multiplexed spectral output of the light passing through the interferometer;
• means operatively connected to the interferometer for selecting known retardations of said movable mirror of said interferometer, wherein said step-scan interferometer maintains the image fidelity of the subject as said fluorescent light passes through the interferometer;
• and
• means for collimating and directing said fluorescent light passing through the interferometer at a focal plane array detector comprising a two-dimensional array of charge coupled devices, wherein said charge coupled devices of said focal plane array detector measures the intensity of emitted light from each of said plurality of spatial locations of the subject at each of a plurality of said known retardations of the movable mirror.

A spectroscopic imaging device employing imaging quality spectral filters suitable for use in near-infrared and infrared absorption microscopy comprising:

• a source of broadband light;
• means for directing said broadband light toward a subject to be analyzed;
• collimation means for directing light transmitted or reflected from each of a plurality of spatial locations within said subject in response to said broadband light source impinging upon said subject at a step-scan interferometer, the step-scan interferometer being of the type which has at least one movable mirror which can be incrementally retarded to produce a multiplexed spectral output of the light passing through the interferometer;
• means operatively connected to the interferometer for selecting known retardations of said movable mirror of said interferometer, wherein said step-scan interferometer maintains the image fidelity of the subject as said transmitted or reflected light passes through the interferometer;
• and
• means for collimating and directing said transmitted or reflected light passing through the interferometer at a focal plane array detector comprising a two-dimensional array of charge coupled devices, wherein said charge coupled devices of said focal plane array detector measures the intensity of said transmitted or reflected light from each of said plurality of spatial locations of the subject at each of a plurality of said known retardations of the movable mirror.