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US 5,374,553 AGranted
DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
AssigneeHoffmann-La Roche Inc.
InventorsDavid H. Gelfand, Frances C. Lawyer
Life of this patent
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Abstract
A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperaturecycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Claims8
An isolated nucleic acid that encodes a thermostable Thermotoga maritima DNA polymerase protein, said protein having the amino acid sequence, from amino to carboxy terminus of:
Dependencies are inferred from the claim text.