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US 5,371,002 AGranted
Method of production of poly-beta-hydroxyalkanoate copolymers
AssigneeJames Madison University
InventorsDouglas E. Dennis, Steven C. Slater
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Abstract
The invention related to recombinant deoxyribonucleic acid (DNA) technology and, more particularly, to a process whereby poly-beta-hydroxyalkanoate (PHA) copolymers can be synthesized in a recombinant host strain containing the poly-beta-hydroxybutyrate (PHB) biosynthetic genes of Alcaligenes eutrophus.
Claims8
A method for producing poly-beta-hydroxyalkanoate copolymers comprising:
- (a) introducing into an Escherichia coli host cell which expresses enzymes of acetate utilization constituitively, a vector containing a DNA sequence coding for a poly-beta-hydroxybutyrate biosynthetic pathway;
- (b) culturing the host cell on a substrate comprising a carbon source and propionate;
- (c) obtaining expression of the poly-betahydroxybutyrate biosynthetic pathway in the host cell;
- and,
- (d) recovering poly-beta-hydroxyalkanoate copolymers produced by the host cell.
2 more elements
Dependencies are inferred from the claim text.