Fatty acyl reductases
AssigneeCalgene, Inc.
InventorsJames G. Metz, Michael R. Pollard, Michael W. Lassner
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Abstract
By this invention, a partially purified seed-plant fatty acyl reductase protein is provided, wherein said protein is active in the formation of a fatty alcohol from a fatty acyl substrate. Of special interest are jojoba embryo reductase proteins having molecular mass of about 54 and 52 kD and sequences obtainable therefrom. Also considered are amino acid and nucleic acid sequences obtainable from such fatty acyl reductases, which sequences may be used for preparation of recombinant constructs useful for expression of reductase in host cells, which results in the production of fatty alcohols in said cells.
Claims26
A recombinant construct comprising a nucleic acid sequence which encodes a jojoba embryo long chain fatty acyl-CoA reductase wherein said reductase is active in the formation of a fatty alcohol from a fatty acyl substrate.
An E. coli cell comprising a jojoba embryo long chain fatty acyl-CoA reductase.
A method of producing a fatty acyl-CoA reductase in a plant or bacterial cell comprising the steps of
- growing a plant or bacterial cell containing a recombinant construct,
- said construct comprising a nucleic acid sequence which encodes a jojoba embryo long chain fatty acyl-CoA reductase wherein said reductase is active in the formation of a fatty alcohol from a fatty acyl substrate and wherein said reductase encoding sequence is under the control of regulatory elements functional in said plant or bacterial cell,
- under conditions which will cause the expression of said reductase sequence.
A method of producing a fatty alcohol in a plant or E. coli cell comprising the steps of
- growing a plant or E. coli cell containing a recombinant construct,
- said construct comprising a nucleic acid sequence which encodes a jojoba embryo long chain fatty acyl-CoA reductase wherein said reductase is active in the formation of a fatty alcohol from a fatty acyl substrate and wherein said reductase encoding sequence is under the control of regulatory elements functional in said plant or E. coli cell,
- wherein said plant or E. coli cell is grown under conditions which will cause the expression of said reductase sequence,
- and wherein said plant or E. coli cell contains a fatty acyl substrate of said reductase.
A recombinant construct comprising a nucleic acid sequence encoding a fatty acyl-CoA reductase active in the formation of a fatty alcohol from a fatty acyl substrate, wherein said nucleic acid sequence encodes a fatty acyl-CoA reductase protein having the amino acid sequence shown in FIG. 1.
Dependencies are inferred from the claim text.