Oligonucleotides with selectably cleavable and/or abasic sites
AssigneeChiron Corporation
InventorsMichael S. Urdea, Thomas Horn
Life of this patent
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Abstract
A modified polynucleotide containing at least one cleavable or abasic site as shown below. ##STR1## DNA.sub.1 is a first segment of DNA; DNA.sub.2 is a second segment of DNA; and R.sub.m is C.sub.1 to C.sub.16 alkylene or an oxytheylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an interger in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR2## Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.
Claims23
A polynucleotide reagent having the structure ##STR30## wherein DNA.sub.1 is a first segment of DNA;
- DNA.sub.2 is a second segment of DNA;
- and
- R.sub.m is C.sub.1 -C.sub.16 alkylene or an oxyethylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an integer in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR31##
Dependencies are inferred from the claim text.