Stable forms of antigenic Taenia ovis polypeptides
AssigneesPitman-Moore New Zealand Limited, The University of Melbourne, Her Majestry the Queen in right of New Zealand through the Ministry of Agriculture & Fisheries
InventorsGavin B. L. Harrison, Robert P. Dempster, Michael D. Rickard, Marshall W. Lightowlers, David D. Heath, Stephen B. Lawrence, Kim L. O'Hoy
Life of this patent
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Abstract
Stable forms of Taenia ovis peptide antigens, such as portions of a T. ovis oncosphere antigen which runs as a 47-52 kDa doublet, are suitable for use in vaccines to protect ruminants against infection by cestode parasites. The antigens are preferably obtained by isolation and expression of the DNA encoding them in recombinant host cells. Aspects of the invention include DNA encoding T. ovis antigens, vectors containing such DNA and host cells which express the T. ovis antigens.
Claims27
A stable antigenic immunologically active protective peptide essentially free from naturally occurring admixtures, said peptide comprising a fragment of a T. ovis oncosphere antigen which runs as a 47-52 kDa doublet on SDS-PAGE, which fragment:
- (a) has a molecular weight of from about 23 kDa to about 24 kDa;
- and
- (b) generates a protective immunological response to T. ovis infection in a ruminant;
- or a stable protective subfragment of said 23-24 kDa fragment which subfragment generates a protective immunological response to T. ovis infection.
An isolated and essentially purified DNA molecule comprising a nucleotide sequence which encodes a purified stable protective antigenic peptide comprising a fragment of a T. ovis polypeptide antigen which runs as a 47-52 kDa doublet on SDS-PAGE which fragment:
- (a) has a molecular weight of from about 23 kDa to about 24 kDa;
- (b) generates a protective immunological response to T. ovis infection in a ruminant;
- or
- which encodes a protective subfragment, said subfragment generating a protective immunological response to T. ovis infection.
An isolated and essentially purified DNA molecule which encodes an immunologically active protective peptide comprising a fragment of a T. ovis polypeptide which fragment is coded for by the nucleotide sequence
- __________________________________________________________________________ 1 CGG ACTACGAACA ACCCATCGAG AGAACAGTGG TAGAATATCC ATCACTACGT 54 GACATCTTTG CTTGGGAACC TCCGACTTCT AACTCCATTG GCCTAACTTG GCAAAGGCAT 114 GCATTTCCTG GTGTGGAACG TGAAGTGCTC ACATTGAAGG CAGTGCCGAC TTCTGAACCC 174 AATAACACCA AGACAGCATA TGCAAAGCTC GGCAGCGGAA AAGTCACTCT TGATGGACTG 234 AAGCCCAATG CCACATATCT TGTGACTGCG ACGGCAAATA TAAGTGGAGA CACAATTCTG 294 GTATTGAGCA ATACTTTTCA TACACTGGCC AATGGCACAA ATATTATAAA TAACATCTTC 354 CATTGGGGTC CTGTGACTAA TCAATCAATT CAAGTAAGAT GGGATCAGAT AAAACCGGAG 414 GAAACAAGCG CTCTGATAGT CACACTGACG GCAGAGATGG CTTCTGACCC CGGAGTGGAA 474 AGATCGGAGT CTGCACTCTT CGGTAAAGGA AAGGTCACTG TTGACGGACT GGAGTCCGAC 534 ACACTATATA TTGCGACTGT GATGGTATTT AGAAATGGAA GGCAATACTT CAATTCCACC 594 AGAGATATTC GAACACTCAA ATCTGGCCAT AAGGAGGTAA CAGTCGTAAC AACTAGTGGA 654 TC __________________________________________________________________________
An isolated and essentially purified DNA molecule comprising a 655 bp fragment of DNA as shown in FIG. 1 contained within E. coli transformant 45 W (ATCC accession number 67507) encoding a stable immunologically active protective peptide comprising a fragment of a polypeptide of T. ovis.
A vector pGEX-2T-45W, consisting essentially of the Bam HI to Xho II fragment of T. ovis DNA as shown in FIG. 1.
Dependencies are inferred from the claim text.