# US 5,365,072 A · Repositionable substrate for microscopes

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- Number: US 5,365,072 A
- Document ID: US5365072A
- Status: Granted
- Kind: A
- Application number: 08/113,126
- Filed: 1993-08-30
- Granted: 1994-11-15
- Earliest claimed: 1993-08-30
- Assignees: The United States of America as represented by the Secretary of the Navy
- Inventors: David C. Turner; Bruce P. Gaber
- Web page: https://patentgrep.com/patent/US5365072A

## CPC

- G01Q 40/02: Calibration standards and methods of fabrication thereof
- G01B 7/004: for measuring coordinates of points
- H01J 37/20: Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
- G01N 2001/2893: {Preparing calibration standards}
- G06V 10/245: {by locating a pattern; Special marks for positioning}
- H01J 2237/20292: Means for position and/or orientation registration
- H01J 2237/30444: Calibration grids
- G01Q: SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01B: MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G06V: IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING

## Abstract

The present invention is a repositionable substrate for microscope applications, having a geometric pattern on its top surface to convey coordinate information. In a preferred embodiment, this geometric pattern is a plurality of geometric shapes having a common axis of symmetry. In a most preferred embodiment, these geometric shapes are concentric, with varying linewidth.

## Claims

### Claim 1

A repositionable substrate for microscopic applications, comprising a substrate having a pattern on its top surface to convey coordinate information, wherein said pattern on said top surface comprises a plurality of concentric geometric shapes centered about an origin point on said top surface, wherein each of said plurality of said geometric shapes comprises one or more lines having a unique linewidth corresponding to a unique distance of said geometric shape from said origin point.

### Claim 2 (depends on 1)

The repositionable substrate of claim 1, wherein each of said one or more lines having a unique linewidth has two opposing edges, wherein the first of said two opposing edges is contoured to be distinguishable from the second of said two opposing edges.

### Claim 3 (depends on 1)

The repositionable substrate of claim 1, wherein said concentric geometric shapes are concentric squares.

### Claim 4 (depends on 1)

The repositionable substrate of claim 1, wherein said concentric geometric shapes are concentric triangles.

### Claim 5 (depends on 1)

The repositionable substrate of claim 1, wherein said concentric geometric shapes are concentric circles.

### Claim 6

A repositionable substrate for microscopic applications, comprising a substrate having a pattern on its top surface to convey coordinate information, wherein said pattern on said top surface comprises a plurality of geometric shapes having at least one common axis of symmetry passing through an origin point on said top surface, wherein each of said plurality of said geometric shapes comprises one or more lines having a unique dimension corresponding to a unique distance of said geometric shape from said origin point.

### Claim 7

A repositionable substrate for microscopic applications, comprising a substrate having a pattern on its top surface to convey coordinate information, wherein said pattern on said top surface comprises a plurality of geometric shapes having at least one common axis of symmetry passing through an origin point on said top surface, wherein each of said plurality of said geometric shapes varies in at least one dimension, wherein said variation in said dimension corresponds to a distance from said origin point.

### Claim 8

A repositionable substrate for microscopic applications, comprising a substrate having a pattern on its top surface to convey coordinate information, wherein said pattern on said top surface comprises a plurality of lines, wherein the direction of each of said lines corresponds to the direction from said line to an origin point on said top surface, and wherein a physical dimension of each of said lines corresponds to the distance from said line to said origin point.