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AssigneeGenencor International, Inc.
InventorsRandy M. Berka, Daniel Cullen, Gregory L. Gray, Kirk J. Hayenga, Virgil B. Lawlis
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Novel vectors are disclosed for expressing and secreting heterologous polypeptides from filamentous fungi. Such vectors are used in novel processes to express and secrete such heterologous polypeptides. The vectors used for transforming a filamentous fungus to express and secrete a heterologous polypeptide include a DNA sequence encoding a heterologous polypeptide and a DNA sequence encoding a signal sequence which is functional in a secretory system in a given filamentous fungus and which is operably linked to the sequence encoding the heterologous polypeptide. Such signal sequences may be the signal sequence normally associated with the heterologous polypeptides or may be derived from other sources. The vector may also contain DNA sequences encoding a promoter sequence which is functionally recognized by the filamentous fungus and which is operably linked to the DNA sequence encoding the signal sequence. Preferably functional polyadenylation sequences are operably linked to the 3' terminus of the DNA sequence encoding the heterologous polypeptides. Each of the above described vectors are used in novel processes to transform a filamentous fungus wherein the DNA sequences encoding the signal sequence and heterologous polypeptide are expressed. The thus synthesized polypeptide is thereafter secreted from the filamentous fungus.
A DNA construct for use in transforming an Aspergillus host to enable secretion of a heterologous polypeptide, said DNA construct comprising promoter DNA from an Aspergillus gene operably linked to coding DNA, said coding DNA comprising DNA coding for a signal peptide and said heterologous polypeptide.
A DNA construct for use in transforming an Aspergillus host to enable secretion therefrom of a polypeptide foreign thereto, said DNA construct comprising promoter DNA operably linked to coding DNA to enable expression thereof, said coding DNA comprising DNA coding for said foreign polypeptide operably linked to DNA coding for a signal peptide enabling said polypeptide to be secreted when said coding DNA is expressed, said promoter DNA and the signal peptide-encoding DNA corresponding in sequence to the glucoamylase gene of Aspergillus.
A transformed Aspergillus expression host capable of secreting a heterologous polypeptide, said host being transformed with a vector comprising promoter DNA from an Aspergillus gene operably linked to coding DNA, said coding DNA comprising DNA coding for a signal peptide and said heterologous polypeptide.
A process for producing a polypeptide comprising:
The process of claim 44 wherein said mammalian polypeptide comprises chymosin or prochymosin.
Dependencies are inferred from the claim text.