Ornithine carbamoyl transferase gene and utilization of the DNA

An isolated DNA sequence comprising the following, operably linked in the 5' to 3' direction:

• a promoter, an OCTase coding region of C. hirsutus;
• and a transcription termination region, wherein the OCTase coding region encodes an amino acid sequence having the amino acid sequence shown in SEQ ID No:
• 1 or SEQ ID No:
• 2.

An isolated recombinant linear or circular DNA comprising:

• (1) an OCTase coding region of C. hirsutus;
• or
• (2) a DNA sequence comprising he following, operably linked in the 5' to 3' direction:
• a promoter, an OCTase coding region of C. hirsutus and a transcription termination region,
• wherein the OCTase coding region encodes an amino acid sequence having the amino acid sequence shown in SEQ ID No:
• 1 or SEQ ID No:
• 2.

An isolated recombinant linear or circular DNA comprising:

• (1) an OCTase coding region of C. hirsutus;
• or a DNA sequence comprising the following, operably linked in the 5' to 3' direction:
• a first promoter, an OCTase coding region of C. hirsutus and a transcription termination region;
• (2) a second promoter operable in a basidiomycete of the genus Coriolus;
• and
• (3) a coding region coding for a desired protein under control by the second promoter, wherein the OCTase coding region encodes an amino acid sequence having the amino acid sequence shown in SEQ ID No:
• 1 or SEQ ID No:2;
• wherein the first promoter may be the same or different from the second promoter.

A host-vector system comprising:

• (1) a DNA sequence comprising the following, operably linked in the 5' to 3' direction:
• a promoter, an OCTase coding region of C. hirsutus and a transcription termination region, wherein the OCTase coding region encodes an amino acid sequence having the amino acid sequence shown in SEQ ID No:
• 1 or SEQ ID No:
• 2;
• and
• (2) an auxotrophic mutant, OJI-1078 (FERM BP-4210), of the genus Coriolus deficient in an ability to express OCTase.

A process for production of a transformant basidiomycete of the genus Coriolus capable of expressing a desired protein, comprising the steps of:

• (a) cotransforming an auxotrophic mutant, OJI-1078 (FERM BP-4210), of a basidiomycete of the genus Coriolus deficient in an ability to express OCTase, with:
• (1) a linear or circular DNA sequence comprising the following, operably linked in the 5' to 3' direction:
• a promoter, an OCTase coding region of C. hirsutus and a transcription termination region, and
• (2) a linear or circular DNA comprising the following, operably linked in the 5' to 3' direction:
• a promoter, a coding region encoding the desired protein, and a transcription termination region;
• and
• (b) selecting a transformant capable of growing in a medium lacking arginine.

A process for production of a transformant basidiomycete of the genus Coriolus capable of expressing a desired protein, comprising the steps of:

• (a) transforming an auxotrophic mutant, OJI-1078 (FERM BP-4210), of a basidiomycete of the genus Coriolus deficient in an ability to express OCTase, with a linear or circular DNA comprising:
• (1) an OCTase coding region of C. hirsutus, or a DNA sequence comprising the following operably linked in the 5' to 3' direction:
• a first promoter, an OCTase coding region of C. hirsutus and a transcription termination region;
• (2) a second promoter operable in a basidiomycete of the genus Coriolus;
• and
• (3) a coding region for the desired protein under the control by the second promoter;
• and
• (b) selecting a transformant capable of growing a medium lacking arginine, wherein the first promoter may be the same as or different from the second promoter.