Stimulation of stem cell growth by the bryostatins
AssigneeThe Johns Hopkins University
InventorsW. Stratford May, Lyle L. Sensenbrenner
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Abstract
In vivo and in vitro methods are taught for stimulating the differentiation of stem cells using certain bryostatins. Stem cells can be induced to form granulocytes, macrophages and erythrocytes. In addition, the cytotoxicity of mature neutrophils can be activated. These methods may substitute for those requiring human colony stimulating factors.
Claims28
A method of stimulating the formation of CFU-GM comprising:
- obtaining a cell preparation containing hematopoietic stem cells;
- and
- incubating the cell preparation in vitro with a bryostatin having an acylated C20 carbon in an amount effective to stimulate the formation of granulocyte-macrophage colonies.
A method of increasing formation of CFU-GM and BFU-E in a mammal comprising:
- administering a bryostatin having an acylated C20 carbon to the mammal in an amount effective to increase the and formation of CFU-GM or BFU-E.
A method of stimulating the formation of CFU-E and BFU-E comprising:
- obtaining a cell preparation containing hematopoietic stem and
- incubating the cell preparation in vitro with erythropoietin and a bryostatin having an acylated C20 carbon in an amount effective to increase the formation of CFU-E and BFU-E.
The method of claim 24, wherein the chemotherapeutic agent is selected from the group consisting of:
- adriamycin, cytosine arabinoside, vincristine, cyclophosphamide, busulfan, prednisone, and M-AMSA.
Dependencies are inferred from the claim text.