Production of recombinant adeno-associated virus vectors

A method for producing recombinant adeno-associated virus comprising the steps of:

• (a) introducing into a cell an adeno-associated virus transducing vector, said vector comprising an Epstein Barr nuclear antigen gene, an Epstein Barr virus latent origin of replication, and exogenous genetic material, said vector lacking functional adeno-associated rep and cap genes;
• thereby producing a recombinant adeno-associated virus producer cell;
• (b) growing said producer cell in a cell growth medium;
• (c) introducing into said producer cell a recombinant adenovirus or herpes virus vector, wherein said adenovirus or herpes virus vector comprises adeno-associated virus rep and cap genes, and said adenovirus or herpes virus vector retains helper functions sufficient to permit adeno-associated virus replication, thereby producing a transfected cell which produces recombinant adeno-associated virus;
• and,
• (d) isolating said recombinant adeno-associated virus.

A cell line comprising an AAV/EBV plasmid vector, said AAV/EBV plasmid vector comprising:

• an Epstein Barr virus nuclear antigen gene;
• an Epstein Barr virus latent origin of replication;
• and
• an adeno-associated virus transducing vector comprising exogenous genetic material and lacking a functional adeno-associated virus rep gene.

A cell line comprising an AAV/EBV plasmid vector, said AAV/EBV plasmid vector comprising:

• an Epstein Barr virus nuclear antigen gene;
• an Epstein Barr virus latent origin of replication;
• and
• an adeno-associated virus transducing vector comprising exogenous genetic material and lacking functional adeno-associated virus rep and cap genes.