# US 5,352,661 A · Bacillus thuringiensis isolate denoted B.t. PS81GG, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin

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- Number: US 5,352,661 A
- Document ID: US5352661A
- Status: Granted
- Kind: A
- Application number: 07/920,085
- Filed: 1992-07-27
- Granted: 1994-10-04
- Earliest claimed: 1988-11-01
- Assignees: Mycogen Corporation
- Inventors: Jewel Payne; August J. Sick; Mark Thompson
- Web page: https://patentgrep.com/patent/US5352661A

## CPC

- C12N 1/205: Bacterial isolates
- C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
- C12R 2001/075: {Bacillus thuringiensis}
- Y10S 435/832: Bacillus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A novel B.t. isolate with activity against lepidopteran insects is disclosed. This isolate is highly active against the beet armyworm. A gene from this isolate has been cloned. The DNA encoding the B.t. toxin can be used to transform various prokaryotic and eukaryotic microbes to express the B.t. toxin. These recombinant microbes can be used to control lepidopteran insects in various environments.

## Claims

### Claim 1

Isolated toxin active against lepidopteran insects consisting essentially of the amino acid sequence shown in SEQ ID NO. 2.

### Claim 2

A method for controlling lepidopteran insects which comprises administering to said insects or to the environment of said insects a microorganism transformed to express a B.t. toxin consisting essentially of the amino acid sequence shown in SEQ ID NO. 2.

### Claim 3 (depends on 2)

A method according to claim 2, wherein said administration is to the rhizosphere.

### Claim 4 (depends on 2)

A method according to claim 2, wherein said administration is to the phylloplane.

### Claim 5

An insecticidal composition comprising insecticide containing substantially intact, treated cells having prolonged pesticidal activity when applied to the environment of a target pest, wherein said insecticide is a polypeptide toxic to lepidopteran insects, is intracellular, and is produced as a result of expression of a transformed microbe capable of expressing the B.t. toxin having the amino acid sequence shown in SEQ ID NO. 2.