# US 5,352,604 A · Alkaline proteolytic enzyme and method of production

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- Number: US 5,352,604 A
- Document ID: US5352604A
- Status: Granted
- Kind: A
- Application number: 08/033,080
- Filed: 1993-03-10
- Granted: 1994-10-04
- Earliest claimed: 1989-08-25
- Assignees: Henkel Research Corporation
- Inventors: Charles R. Wilson; Beth F. Ladin; Jonathan R. Mielenz; Sherman S. M. Hom; Dieter Hansen; Robert B. Reynolds; Nicholas C. T. Kennedy; Joachim Schindler; Michael Bahn; Rolf Schmid; Martina Markgraf; Christian Paech; Karlheinz Maurer
- Web page: https://patentgrep.com/patent/US5352604A

## CPC

- C12N 9/54: bacteria being Bacillus
- C12N 15/75: for Bacillus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.

## Claims

### Claim 1

A composition comprising a mature alkaline proteolytic enzyme from Bacillus lentus DSM5483 and at least one other proteolytic enzyme from Bacillus licheniformis ATCC 53926, wherein the amino acid residues of said enzyme from Bacillus lentus DSM 5483 at positions 208, 210, 212, 213 and 268 are identical with those of FIG. 1 and wherein the remaining amino acid residues of said enzyme share no less than 95% homology therewith, and with at least one other mature protease of Bacillus.

### Claim 2

A composition comprising a mature alkaline proteolytic enzyme having the amino acid sequence substantially as shown in FIG. 29 and at least one other proteolytic enzyme from Bacillus licheniformis ATCC 53926 wherein the amino acid residues of said enzyme substantially similar to that represented in FIG. 29 are identical with those of FIG. 29 at position 3, 97, 99, 102 and 157 and wherein the remaining amino acid residues share no less than 95% homology therewith, and at least one other mature protease of Bacillus licheniformis ATCC 53926.

### Claim 3 (depends on 1)

A composition of claim 1 further comprising a surfactant.

### Claim 4 (depends on 3)

A composition of claim 3 wherein said surfactant is selected from the group consisting of a linear alkyl benzene sulfonate, an ethoxylated linear alcohol, an alkyl ethoxylated sulfate, a sulfated linear alcohol, or combinations thereof.