# US 5,352,602 A · Isoamylase and process for producing the same

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- Number: US 5,352,602 A
- Document ID: US5352602A
- Status: Granted
- Kind: A
- Application number: 08/024,072
- Filed: 1993-03-01
- Granted: 1994-10-04
- Earliest claimed: 1992-02-28
- Assignees: Amano Pharmaceutical Co., Ltd.
- Inventors: Yuzo Yamada; Toshihiro Sato; Takaichi Ohya
- Web page: https://patentgrep.com/patent/US5352602A

## CPC

- C12N 9/246: {Isoamylase (3.2.1.68)}
- C12Y 302/01068: Isoamylase (3.2.1.68)
- Y10S 435/91: Xanthomonas
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

An isoamylase, useful in the field of starch saccharification, having a molecular weight of about 105,000, an isoelectric point of 6.4, an optimum pH of 3.0 to 5.0, an optimum temperature of about 50.degree. C. and exhibiting temperature stability at 45.degree. C..times.10 minutes and pH stability at pH 3.5 to 6.0, and a process for producing the same comprising cultivating an isoamylase-producing strain belonging to the genus Xanthomonas and recovering the enzyme produced.

## Claims

### Claim 1

Substantially pure isoamylase produced by cultivating an isoamylase-producing Xanthomonas maltophilia strain, said substantially pure isoamylase having the following enzymological and chemical properties:
1) Substrate specificity: actively acts on glycogen, also acts on amylopectin, hardly acts on pullulan,
2) Optimum pH: 3.0 to 5.0,
3) pH Stability: pH 3.5 to 6.0,
4) Optimum Temp.: around 50.degree. C.,
5) Temperature Stability: stable at 45.degree. C. for 10 minutes,
6) Isoelectric point: 6.4, and
7) Molecular weight: about 105,000, as measured by gel-filtration on SUPEROSE 12.

### Claim 2

A process for producing an isoamylase having the following enzymological and chemical properties:
1) Substrate specificity: actively acts on glycogen, also acts on amylopectin, hardly acts on pullulan,
2) Optimum pH: 3.0 to 5.0,
3) pH Stability: pH 3.5 to 6.0,
4) Optimum Temp.: around 50.degree. C.,
5) Temperature Stability: stable at 45.degree. C. for 10 minutes,
6) Isoelectric point: 6.4, and
7) Molecular weight: about 105,000, as measured by gel-filtration on SUPEROSE 12,
comprising cultivating an isoamylase-producing strain belonging to the genus Xanthomonas and recovering the isoamylase produced.

### Claim 3 (depends on 2)

The process of claim 2, wherein the isoamylase-producing strain belonging to the genus Xanthomonas is Xanthomonas maltophilia.

### Claim 4 (depends on 1)

The substantially pure isoamylase of claim 1, wherein the strain of Xanthomonas maltophilia is Xanthomonas maltophilia S-517.

### Claim 5 (depends on 3)

The process of claim 3, wherein the strain of Xanthomonas maltophilia is Xanthomonas maltophilia S-517.

### Claim 6 (depends on 4)

The substantially pure isoamylase of claim 4, wherein the strain of Xanthomonas maltophilia S-517 is that having accession number FERM BP-4205.

### Claim 7 (depends on 5)

The process of claim 5, wherein the strain of Xanthomonas maltophilia S-517 is that having accession number FERM BP-4205.