# US 5,352,589 A · Deletion mutant of basic fibroblast growth factor and production thereof

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- Number: US 5,352,589 A
- Document ID: US5352589A
- Status: Granted
- Kind: A
- Application number: 08/071,046
- Filed: 1993-06-02
- Granted: 1994-10-04
- Earliest claimed: 1988-09-16
- Assignees: Farmitalia Carlo Erba S.p.A.
- Inventors: Laura Bergonzoni; Antonella Isacchi; Paolo Sarmientos; Gilles Cauet
- Web page: https://patentgrep.com/patent/US5352589A

## CPC

- C07K 14/503: {basic FGF [bFGF]}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

The present invention relates to the production, by recombinant DNA techniques, of derivatives of basic fibroblast growth factor (bFGF). These derivatives of bFGF can act as antagonists and/or superagonists of the wild type molecule in the angiogenic process. These derivatives, as well as wild type bFGF, may be prepared by the use of strains or E. coli which have been transformed with plasmids carrying nucleotide sequence coding for human and bovine bFGF and their derivatives.

## Claims

### Claim 1

A human or bovine basic fibroblast growth factor derivative wherein amino acid residues 27 through 32 (Lys-Asp-Pro-Lys-Arg-Leu) have been deleted, the numbering of the above amino acid residues corresponding to the 155-amino acid form of human or bovine basic FGF.

### Claim 2 (depends on 1)

A human or bovine basic fibroblast growth factor of claim 1 having an N-terminus selected from the naturally occurring microheterogeneous N-termini of human or bovine basic fibroblast growth factors.

### Claim 3 (depends on 1, 2)

A method for the production recombinant derivatives of human or bovine bFGF comprising producing a peptide of claim 1 or 2 an E. coli type B strain.