# US 5,352,576 A · Method of determining the presence and titers of anti HIV-1 ag27bodies

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- Number: US 5,352,576 A
- Document ID: US5352576A
- Status: Granted
- Kind: A
- Application number: 07/931,416
- Filed: 1992-08-24
- Granted: 1994-10-04
- Earliest claimed: 1987-09-08
- Assignees: Duke University
- Inventors: Barton F. Haynes; Thomas J. Palker
- Web page: https://patentgrep.com/patent/US5352576A

## CPC

- C07K 14/005: from viruses
- C07K 17/06: attached to the carrier via a bridging agent
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C07K 2319/40: containing a tag for immunodetection, or an epitope for immunisation
- C12N 2740/16022: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2740/16122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- Y10S 435/974: Aids related test
- Y10S 930/221: Retrovirus related, or human immunodeficiency virus related, or simian immunodeficiency virus related
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Disclosed is a method for determining the presence in mammalian serum of antibodies against specific strains of HIV through the use of SP-10 peptides. The method incorporates the use of SP-10 peptides derived from the following HIV strains: IIIB, MN, RF, SC, WMJ-1, WMJ-2, WMJ-3, ARV-2, and LAV-1.

## Claims

### Claim 1

A method of determining the presence in mammalian serum of antibodies against a specific strain of HIV-1 comprising the steps of: (i) contacting a SP-10 peptide of said HIV-1 strain with said mammalian serum under conditions such that a complex between said peptide and said antibodies present in said mammalian serum can form; and (ii) measuring the formation of said complex.

### Claim 2 (depends on 1)

A method according to claim 1 wherein said peptide is selected from the group consisting of SP-10 IIIB, SP-10 MN, SP-10 RF, SP-10 SC, SP-10 WMJ-1, SP-10 WMJ-2, SP-10 WMJ-3, SP-10 ARV-2 and SP-10-LAV-I.