# US 5,352,575 A · Pseudorabies virus protein

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- Number: US 5,352,575 A
- Document ID: US5352575A
- Status: Granted
- Kind: A
- Application number: 07/513,282
- Filed: 1990-04-20
- Granted: 1994-10-04
- Earliest claimed: 1985-10-04
- Assignees: The Upjohn Company
- Inventors: Erik A. Petrovskis; Leonard E. Post; James G. Timmins
- Web page: https://patentgrep.com/patent/US5352575A

## CPC

- C07K 14/005: from viruses
- G01N 33/56983: {Viruses}
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- C12N 2710/16722: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- Y10S 435/975: Kit
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

The present invention provides recombinant DNA molecules comprising a sequence encoding a pseudorabies virus (PRV) glycoprotein selected from the group consisting of gI, gp50, and gp63, host cells transformed by said recombinant DNA molecule sequences, the gI, gp50 and gp63 polypeptides. The present invention also provides subunit vaccines for PRV, methods for protecting animals against PRV infection and methods for distinguishing between infected and vaccinated animals.

## Claims

### Claim 1

A method for distinguishing an animal vaccinated with a pseudorabies virus (PRV) vaccine lacking glycoprotein gI or gp63 from an animal infected with a virulent wild-type pseudorabies virus without sacrificing the animal comprising: contacting serum from the animal with PRV glycoprotein gI or gp63, and detecting the presence or absence of antibodies which specifically bind to PRV glycoprotein gI or gp63 wherein the presence of antibodies indicates that the animal has been infected with a virulent wild-type pseudorabies virus.

### Claim 2 (depends on 1)

The method of claim 1 wherein the animal has been vaccinated with a PRV vaccine lacking glycoprotein gI and the serum is contacted with PRV glycoprotein gI.

### Claim 3 (depends on 1)

The method of claim 1 wherein the presence or absence of the antibodies is detected using an ELISA.

### Claim 4 (depends on 1)

A mercantile kit useful in performing the method of claim 1, comprising multiple containers wherein one of said containers has therein PRV glycoprotein gI or gp63 and another of said containers has therein a reagent for detection of antibodies which specifically bind to gI or gp63.

### Claim 5 (depends on 4)

The mercantile kit of claim 4 wherein the PRV glycoprotein is gI.