# US 5,352,574 A · Surface functionalized and derivatized electroactive polymers with immobilized active moieties > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 5,352,574 A - Document ID: US5352574A - Status: Granted - Kind: A - Application number: 07/771,759 - Filed: 1991-10-04 - Granted: 1994-10-04 - Earliest claimed: 1989-03-13 - Inventors: Anthony Guiseppi-Elie - Web page: https://patentgrep.com/patent/US5352574A ## CPC - G01N 27/126: {comprising organic polymers} - C08F 8/00: Chemical modification by after-treatment (graft polymers, block polymers, crosslinking with unsaturated monomers or with polymers C08F251/00 - C08F299/00; of conjugated diene rubbers C08C) - C08F 8/12: Hydrolysis - C12Q 1/002: {Electrode membranes} - C12Q 1/003: {Functionalisation} - C12Q 1/005: {involving specific analytes or enzymes (including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence)} - B01J 2219/00653: the compounds being bound to electrodes embedded in or on the solid supports - B01J 2219/00659: Two-dimensional arrays - Y10S 435/817: Enzyme or microbe electrode - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - C08F: MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract New variants of electroactive and optoactive polymers are formed from the surface chemical modification and derivization of free-standing and substrate-supported polymer films. The free-standing or substrate-supported films are chemically modified at or near their surfaces to introduce hydrophilic and/or reactive functional groups, such as carboxylic acids, hydroxyls, and amines. Surface derivatization of the modified polymer film is achieved through the specific attachment of bioactive, immunoactive, electroactive, and catalytic agents to the surface of the electroactive or optoactive polymer film. In one embodiment, a polymer selected from polyacetylene, polypyrrole, polyanilane and polythiophene is modified to contain functional groups and an indicator reagent is covalently coupled to the functional groups. When an analyte in a sample reacts with the indicator reagent, electrical conductivity of the polymer is changed and presence of the analyte is indicated by the change in electrical conductivity. ## Claims ### Claim 1 A process for the immobilization of an indicator reagent upon a chemically modified surface of an electroactive polymeric substrate having a predetermined electrical conductivity, the process comprising: (a) reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene with at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate, the activated surface being associated with a predetermined electrical conductivity; (b) reacting the activated surface on the substrate with a linking agent; and (c) reacting the linking agent with an indicator reagent to covalently band the indicator reagent to the activated surface of the substrate such that the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent. ### Claim 2 (depends on 1) The process of claim 1 wherein the electroactive polymer is polyacetylene and the method of surface activation comprises the formation of surface hydroxyl groups. ### Claim 3 (depends on 1) The process of claim 1 wherein the electroactive polymer is polyacetylene and the linking agent is p-benzoquinone. ### Claim 4 (depends on 3) The process of claim 3 wherein the indicator reagent is glucose oxidase. ### Claim 5 (depends on 1) The process of claim 1 wherein the electroactive polymer is polyanaline and the method of surface activation comprises immersion of the polymer in a mixed sulphuric:nitric acid solution and said activated surface comprises amino groups. ### Claim 6 (depends on 1) The process of claim 1 wherein the electroactive polymer polyanaline and the linking agent is carbodiimide. ### Claim 7 (depends on 6) The process of claim 6 wherein the indicator reagent is flavin adenine dinucleotide. ### Claim 8 (depends on 1) The process of claim 1 wherein the electroactive polymer is polyanaline and the linking agent is p-benzoquinone. ### Claim 9 (depends on 8) The process of claim 8 wherein the indicator reagent glucose oxidase. ### Claim 10 A method for detecting the presence of an analyte in a sample comprising contacting a sample with a material comprising an electroactive polymeric substrate having a predetermined electrical conductivity containing a covalently bound indicator reagent, reacting analyte in the sample with the indicator reagent to change the electrical conductivity of the suffererate and from the change in the electrical conductivity determining the presence of the analyte, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity at least one chemical modification reagent to produce an activated surface containing new functional groups capable of covalent bonding on the surface of the substrate and having a predetermined electrical conductivity, and reacting the new functional groups of the activated surface with an indicator reagent to covalently bond the indicator reagent to the surface of the substrate. ### Claim 11 A material comprising; an electroactive polymeric substrate containing a covalently bound indicator reagent, said material being prepared by reacting an electroactive polymeric substrate selected from the group consisting of polyacetylene, polypyrrole, polyaniline and polythiophene having a predetermined electrical conductivity with at least one chemical modification reagent to produce an activated surface on the substrate containing new functional groups capable of covalent bonding on the surface of said substrate and said activated surface having a predetermined electrical conductivity, reacting said activated surface with a linking agent, and reacting the linking agent with an indicator reagent to covalently bond the indicator reagent to the activated surface of said substrate, whereby the predetermined electrical conductivity of the substrate can be altered by reactions of an analyte with the indicator reagent.