# US 5,352,476 A · Nutrient composition containing non-phosphorylated peptides from case in based material

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- Number: US 5,352,476 A
- Document ID: US5352476A
- Status: Granted
- Kind: A
- Application number: 07/872,357
- Filed: 1992-04-23
- Granted: 1994-10-04
- Earliest claimed: 1980-02-01
- Assignees: Institut National de la Recherche Agronomique
- Inventors: Gerard Brule; Loic Roger; Jacques Fauquant; Michel Piot
- Web page: https://patentgrep.com/patent/US5352476A

## CPC

- A23J 3/344: {of casein}
- A23C 9/1422: {by ultrafiltration, microfiltration or diafiltration of milk, e.g. for separating protein and lactose; Treatment of the UF permeate}
- A23J 7/00: Phosphatide compositions for foodstuffs, e.g. lecithin
- A61K 38/018: {from milk}
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/14: for calcium homeostasis (vitamin D A61P3/02; parathyroid hormones A61P5/18; calcitonin A61P5/22; osteoporosis A61P19/10; bone metastasis A61P35/04)
- Y10S 530/832: Milk; colostrum
- A23J: PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23C: DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF (preservation thereof A23B11/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Non-phosphorylated peptides and phosphopeptides useful as alimentary products or medicaments are produced by proteolytic enzyme hydrolysis of a casein-based material. Membrane ultrafiltration is used to separate phosphopeptides which are retained by the membrane in a retentate from the non-phosphorylated peptides which pass through the membrane in an ultrafiltrate. The phosphopeptides in the retentate are disaggregated and are subjected to further membrane ultrafiltration where the phosphopeptides pass through the membrane and are separated from the enzyme. The non-phosphorylated peptides have nutritive value and can be used to form compositions for providing nutrition. The phosphopeptides form salts, which have dietetic uses, with macroelements such as calcium and/or magnesium and/or oligoelements such as iron and zinc.

## Claims

### Claim 1

A composition for effecting nutrition, which comprises:
a) an amount of an essentially pure casein non-phosphorylated peptide sufficient to effect nutrition, which comprises greater than 12% by weight of aromatic amino acids, less than 4% per weight of serine, less than 10% by weight of free amino acids, and a value of less than 0.02 the formula: ##EQU2## wherein Ca is total calcium, Mg is total magnesium, P is total phosphorus, and N.sub.T is total nitrogen .times.6.38; and wherein said non-phosphorylated peptide has values of N.sub.T, Ca and P in the range of 27.0 to 53.3 g/kg, 0.14 to 0.20 g/kg and 0.35 to 0.40 g/kg, respectively; and
b) a carrier suitable for consumption.