# US 5,352,458 A · Tanning method using DNA repair liposomes

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- Number: US 5,352,458 A
- Document ID: US5352458A
- Status: Granted
- Kind: A
- Application number: 07/995,262
- Filed: 1992-12-21
- Granted: 1994-10-04
- Earliest claimed: 1992-12-21
- Assignees: Applied Genetics, Inc.
- Inventors: Daniel B. Yarosh
- Web page: https://patentgrep.com/patent/US5352458A

## CPC

- A61K 8/66: Enzymes
- A61K 8/14: Liposomes; Vesicles
- A61Q 19/04: for chemically tanning the skin (topical sun or radiation screening or tanning preparations A61Q17/04)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A method for tanning skin is provided in which liposomes containing a DNA repair enzyme are administered to skin in combination with exposure of the skin to UV radiation. The result is an enhanced level of melanin production, i.e., more tanning than achieved by UV radiation alone. The administration of the DNA repair enzymes in liposomes also reduces the level of DNA damage caused by the UV exposure. Accordingly, both the tanning response is increased and the deleterious effect of UV exposure is decreased. The method can be used by the general population as well as by individuals whose skin is susceptible to UV-induced damage.

## Claims

### Claim 1

A method for tanning skin comprising applying liposomes containing at least one DNA repair enzyme to skin in an amount effective to produce tanning or melanin production and exposing the skin to UV radiation, said applying of the liposomes occurring before and/or during and/or after the exposure to said UV radiation.

### Claim 2 (depends on 1)

The method of claim 1 wherein the liposomes are applied to the skin before exposure to UV radiation.

### Claim 3 (depends on 1)

The method of claim 1 wherein the liposomes are applied to the skin during exposure to UV radiation.

### Claim 4 (depends on 1)

The method of claim 1 wherein the liposomes are applied to the skin after exposure to UV radiation.

### Claim 5 (depends on 1)

The method of claim 1 wherein the skin is repetitively exposed to UV radiation.

### Claim 6 (depends on 1)

The method of claim 1 wherein the liposomes are suspended in a hydrogel.

### Claim 7 (depends on 1)

The method of claim 1 wherein the at least one DNA repair enzyme comprises T4 endonuclease V.

### Claim 8 (depends on 1)

The method of claim 1 wherein the at least one DNA repair enzyme comprises a photolyase.

### Claim 9

A method for inducing melanin production by melanocytes comprising administering liposomes containing at least one DNA repair enzyme in an amount effective to induce melanogenesis to melanocytes and exposing the melanocytes to UV radiation, said administering of the liposomes occurring before and/or during and/or after the exposure to said UV radiation.

### Claim 10 (depends on 9)

The method of claim 9 wherein the liposomes are to the melanocytes before exposure to UV radiation.

### Claim 11 (depends on 9)

The method of claim 9 wherein the liposomes are administered to the melanocytes during exposure to UV radiation.

### Claim 12 (depends on 9)

The method of claim 9 wherein the liposomes are administered to the melanocytes after exposure to UV radiation.

### Claim 13 (depends on 9)

The method of claim 9 wherein the melanocytes are repetitively exposed to UV radiation.

### Claim 14 (depends on 9)

The method of claim 9 wherein the at least one DNA repair enzyme comprises T4 endonuclease V.

### Claim 15 (depends on 9)

The method of claim 9 wherein the at least one DNA repair enzyme comprises a photolyase.

### Claim 16

A method for protecting the skin of an individual susceptible to UV-induced damage comprising applying liposomes containing at least one DNA repair enzyme to the individual's skin in an amount effective to produce tanning or melanin production and exposing the individual's skin to UV radiation whereby the individual's skin becomes tanned, said applying of the liposomes occurring before and/or during and/or after the exposure to said UV radiation.

### Claim 17 (depends on 16)

The method of claim 16 wherein the liposomes are applied to the skin before exposure to UV radiation.

### Claim 18 (depends on 16)

The method of claim 16 wherein the liposomes are applied to the skin during exposure to UV radiation.

### Claim 19 (depends on 16)

The method of claim 16 wherein the liposomes are applied to the skin after exposure to UV radiation.

### Claim 20 (depends on 16)

The method of claim 16 wherein the skin is repetitively exposed to UV radiation.

### Claim 21 (depends on 16)

The method of claim 16 wherein the liposomes are suspended in a hydrogel.

### Claim 22 (depends on 16)

The method of claim 16 wherein the at least one DNA repair enzyme comprises T4 endonuclease V.

### Claim 23 (depends on 16)

The method of claim 16 wherein the at least one DNA repair enzyme comprises a photolyase.