# US 5,350,690 A · Sucrose inducible expression systems

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- Number: US 5,350,690 A
- Document ID: US5350690A
- Status: Granted
- Kind: A
- Application number: 07/876,728
- Filed: 1992-04-28
- Granted: 1994-09-27
- Earliest claimed: 1987-06-19
- Assignees: Amgen Inc.
- Inventors: Mark M. Zukowski
- Web page: https://patentgrep.com/patent/US5350690A

## CPC

- C12N 15/75: for Bacillus
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

This disclosure relates to a sucrose inducible expression system. The sucrose inducible expression system comprises a novel plasmid vector having a sacY gene of the sacS locus which binds to a transcription terminator to relieve transcription termination when introduced into a B. subtilis host microorganism.

## Claims

### Claim 1

A plasmid vector for expressing a gene encoding a desired polypeptide in a B. subtilis host microorganism, said vector being selected from the group consisting of pA51S and pAMB12T5S.

### Claim 2 (depends on 1)

A B. subtilis strain transformed with a vector according to claim 1.

### Claim 3 (depends on 1)

A B. subtilis culture capable of producing a polypeptide by expression of a gene coding therefor, wherein the expression is directed by a plasmid vector according to claim 1.