# US 5,350,683 A · DNA encoding type II interleukin-1 receptors

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- Number: US 5,350,683 A
- Document ID: US5350683A
- Status: Granted
- Kind: A
- Application number: 08/091,519
- Filed: 1993-07-12
- Granted: 1994-09-27
- Earliest claimed: 1990-06-05
- Assignees: Immunex Corporation
- Inventors: John E. Sims; David J. Cosman; Stephen D. Lupton; Bruce A. Mosley; Steven K. Dower
- Web page: https://patentgrep.com/patent/US5350683A

## CPC

- C07K 14/7155: {for interleukins [IL]}
- A61K 38/00: Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

Type II IL-1 receptor (type II IL-1R) proteins, DNAs and expression vectors encoding type II IL-1R, and processes for producing type II IL-1R as products of recombinant cell culture, are disclosed.

## Claims

### Claim 1

An isolated DNA selected from the group consisting of:
(a) a cDNA clone having a nucleotide sequence encoding an amino acid sequence of amino acids 1 through 385 of SEQ ID NO.:2;
(b) a DNA capable of hybridization to a clone of (a) under moderately stringent conditions and which encodes a biologically active type II IL-1R molecule; and
(c) a DNA having a sequence which is degenerate as a result of the genetic code to a DNA as defined in (a) or (b) above and which encodes biologically active type II IL-1R molecules.

### Claim 2 (depends on 1)

An isolated DNA according to claim 1 which encodes a soluble human type II IL-1R.

### Claim 3 (depends on 1)

An isolated DNA according to claim 1 encoding the type II IL-1R polypeptide expressed by pHuIL-1R-II 75 (ATCC 68337).

### Claim 4 (depends on 1)

A recombinant expression vector comprising a DNA according to claim 1.

### Claim 5 (depends on 2)

A recombinant expression vector comprising a DNA according to claim 2.

### Claim 6 (depends on 3)

A recombinant expression vector comprising a DNA according to claim 3.

### Claim 7 (depends on 4)

A process for preparing a type II IL-1R, comprising culturing a suitable host cell comprising a vector according to claim 4 under conditions promoting expression.

### Claim 8 (depends on 5)

A process for preparing a type II IL-1R, comprising culturing a suitable host cell comprising a vector according to claim 5 under conditions promoting expression.

### Claim 9 (depends on 6)

A process for preparing a type II IL-1R, comprising culturing a suitable host cell comprising a vector according to claim 6 under conditions promoting expression.