# US 5,350,677 A · Method of assaying phosphatase with 3,4-dinitrophenylphosphate

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- Number: US 5,350,677 A
- Document ID: US5350677A
- Status: Granted
- Kind: A
- Application number: 08/060,366
- Filed: 1993-05-11
- Granted: 1994-09-27
- Earliest claimed: 1989-02-23
- Assignees: Iatron Laboratories, Inc.
- Inventors: Tatsuhiko Yagi; Ryuki Hisada; Hideto Shibata
- Web page: https://patentgrep.com/patent/US5350677A

## CPC

- C12Q 1/42: involving phosphatase
- C12Q 1/34: involving hydrolase
- C12Q 2334/00: O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q 2334/10: p-Nitrophenol derivatives
- G01N 2333/924: acting on glycosyl compounds (3.2)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The substrate, 3,4-dinitrophenylphosphate, has been found to be useful in an assay of acid phosphatase in body fluids.

## Claims

### Claim 1

A method of assaying an enzyme activity of acidic phosphatase or alkaline phosphatase from a living body comprising:
allowing a sample to be assayed derived from a living body fluid to react with a substrate solution comprising 3,4-dinitrophenylphosphoric acid or a salt thereof; and
measuring a color indication of the 3,4-dinitrophenol formed from the substrate.

### Claim 2 (depends on 1)

A method of assaying an enzyme activity of acidic phosphatase or alkaline phosphatase from a living body as claimed in claim 1 wherein cyclodextrin is also present in the substrate solution.