# US 5,350,672 A · Specific DNA primers and method to use same detect Eperythrozoon suis

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- Number: US 5,350,672 A
- Document ID: US5350672A
- Status: Granted
- Kind: A
- Application number: 08/009,261
- Filed: 1993-01-22
- Granted: 1994-09-27
- Earliest claimed: 1993-01-22
- Assignees: Kansas State University Research Foundation
- Inventors: Richard D. Oberst; Sharon M. Gwaltney; Michael P. Hays
- Web page: https://patentgrep.com/patent/US5350672A

## CPC

- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 1/6893: for protozoa
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The use of E. suis specific primers in PCR with DNA from swine blood increases the sensitivity of current DNA hybridization protocols for determining whether swine are infected with E. suis prior to the development of any clinical symptoms. The present invention provides these E. suis primers and a method to use these primers in a PCR protocol to provide a highly sensitive diagnostic assay for early signs of an E. suis infection.

## Claims

### Claim 1

A method of identifying early Eperythrozoon suis infection in pig blood comprising: isolating DNA from pig blood, contacting said DNA and with oligonucleotide primers consisting of the two single stranded oligonucleotide TCTTCAACTCTTCCTATGGA (SEQ ID NO: 1) and CTCATGGTAAGTTGTGTTGC (SEQ ID NO: 2), amplifying said DNA by polymerase chain reaction, and detecting an amplification product; wherein the presence of the amplification product indicates an early Eperythrozoon suis infection.

### Claim 2

A synthetic oligonucleotide useful in detecting early Eperythrozoon suis infected blood by specifically priming an eperythrozoon specific polymerase chain reaction having the DNA sequence TCTTCAACTCTTCCTATGGA (SEQ ID NO: 1).

### Claim 3

A synthetic oligonucleotide useful in detecting early Eperythrozoon suis infected blood by specifically priming an eperythrozoon specific polmerase chain reaction having the DNA sequence CTCATGGTAAGTTGTGTGTTGC (SEQ ID NO: 2).

### Claim 4

A double stranded DNA amplification product having the DNA sequence set out in SEQ ID NO: 3 on one strand and SEQ ID NO: 4 on the other strand.