# US 5,350,576 A · Bacillus thuringiensis isolates for controlling acarides

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- Number: US 5,350,576 A
- Document ID: US5350576A
- Status: Granted
- Kind: A
- Application number: 08/063,170
- Filed: 1993-05-17
- Granted: 1994-09-27
- Earliest claimed: 1991-09-13
- Assignees: Mycogen Corporation
- Inventors: Jewel Payne; Raymond J. C. Cannon; Angela L. Ralph
- Web page: https://patentgrep.com/patent/US5350576A

## CPC

- C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- Y10S 435/832: Bacillus
- Y10S 530/825: Bacteria
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Disclosed are Bacillus thuringiensis isolates designated B.t. PS45B1, B.t. PS24J, B.t. PS94R3 B.t. PS17, B.t. PS62B1 and B.t. PS74G1 which produce novel .delta.-endotoxins active against acarid pests. Thus, these isolates, or mutants thereof, can be used to control such pests. Claimed are genes encoding these novel .delta.-endotoxins, which can be removed from these isolates and transferred to other host microbes, or plants. Expression of the toxins in microbe hosts results in the control of acarid pests, whereas transformed plants become resistant to acarid pests.

## Claims

### Claim 1

A process for controlling house dust mites (Dermatophagodes pteronyssinus) which comprises contacting said pests with an acarid-controlling effective amount of a Bacillus thuringiensis isolate selected from the group consisting of B.T. OS45B1, B.t. PS24J, G.t. PS94R3, B.t. PS17, B.t. PS62B1 and B.t. PS74G1, and toxins or mutants thereof which retain the property of activity against house dust mites.

### Claim 2 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS45B1.

### Claim 3 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS24J.

### Claim 4 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS94R3.

### Claim 5 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS17.

### Claim 6 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS62B1.

### Claim 7 (depends on 1)

The process, according to claim 1, wherein said microbe is Bacillus thuringiensis PS74G1.

### Claim 8

A method for controlling house dust mites which comprise contacting said pests with an acarid-controlling effective amount of a stable lysis.sup.- spo.sup.- cry.sup.+ Bacillus thuringiensis isolate selected from the group consisting of B.t. PS45B1, B.t. PS24J, B.t. PS94R3 B.t. PS62B1 and B.t. PS74G1, and mutants thereof which retain the property of activity against acarid pests.