# US 5,350,552 A · Method for preparing polyacrylamide aqueous gel plate for electrophoresis

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- Number: US 5,350,552 A
- Document ID: US5350552A
- Status: Granted
- Kind: A
- Application number: 08/009,044
- Filed: 1993-01-26
- Granted: 1994-09-27
- Earliest claimed: 1992-02-07
- Assignees: Daiichi Pure Chemicals Co., Ltd.
- Inventors: Nobuyoshi Ebata; Kazuaki Notsu; Akiko Udagawa; Mieko Shiratori
- Web page: https://patentgrep.com/patent/US5350552A

## CPC

- G01N 27/44704: {Details; Accessories}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A batch method for preparing polyacrylamide aqueous gels for electrophoresis, including providing a plurality of gel plate supporters abreast in an airtight gel-forming container, eliminating oxygen from or introducing oxygen-free gas into said container, charging a gel-forming solution into said container, and gelatinizing the solution. It can easily produce a large quantity of high quality polyacrylamide gel plates for electrophoresis with a homogeneous quality and a high resolving power in an automated batch method in a short period of time.

## Claims

### Claim 1

A batch method for preparing a polyacrylamide aqueous gel plate for electrophoresis comprising:
(1) placing a plurality of gel plate supporters in a gel-forming container which comprises:
(a) a partition rack comprised of plastic or rubber partition members which separate the gel plate supporters from each other,
(b) an inlet port,
(c) an outlet port, and
(d) an airtight lid;
(2) closing the airtight lid;
(3) eliminating oxygen from the container by evacuating air through the outlet port;
(4) charging a gel-forming solution into the container through the inlet port;
(5) allowing the gel-forming solution to gelatinize on the gel plate supporters;
(6) removing the partition rack from the container; and
(7) removing the individual gel plates from the partition rack.

### Claim 2 (depends on 1)

A batch method according to claim 1 wherein the partition members are comprised of polystyrene, styrene foam or fluorinated resin.

### Claim 3 (depends on 2)

A batch method according to claim 2 wherein the partition members are in the form of plates.

### Claim 4 (depends on 1)

A batch method according to claim 1 wherein the partition members are of sufficient height and thickness to prevent adherence of adjacent gel plate supporters and to provide a sufficient insulation effect to suppress generation of heat of polymerization.