# US 5,350,520 A · Method for collecting the components of a sample separated by chromatography

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,350,520 A
- Document ID: US5350520A
- Status: Granted
- Kind: A
- Application number: 08/144,607
- Filed: 1993-11-01
- Granted: 1994-09-27
- Earliest claimed: 1991-04-25
- Assignees: Shimadzu Corporation
- Inventors: Mamoru Kikumoto
- Web page: https://patentgrep.com/patent/US5350520A

## CPC

- G01N 30/82: Automatic means therefor
- G01N 30/80: Fraction collectors
- G01N 30/8631: {Peaks}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Method and apparatus for separately collecting the components of a sample separated by chromatography, which uses a time schedule starting from a specific peak on the chromatogram of a sample to be analyzed and comprising those time bands of a first group in each of which peaks are to be detected and those time bands of a second group in each of which peaks are to be neglected, the time bands of the first and second groups occurring alternately and successively, and only in the time bands of the first group collection of a sample component is conducted while a peak is being detected.

## Claims

### Claim 1

A method of collecting components of a sample separated by chromatography, comprising:
setting a time schedule which starts from a specific peak on a chromatogram of a sample whose components are to be separately collected;
providing said time schedule with a series of time bands of a first group, in each of which peaks on said chromatogram are to be detected for possible utilization in controlling collecting of said sample components, and a series of time bands of a second group, in each of which peaks on said chromatogram are to be neglected, said time bands of said first and second groups occurring alternately and successively;
using a units of time setting in selecting a time length of each of said time bands, individually;
introducing said sample into a chromatograph;
detecting said specific peak to start said time schedule;
detecting at least one peak on the chromatogram of said sample in at least one of said time bands of said first group;
disregarding any peaks on the chromatogram of said sample occurring in said time bands of said second group; and
collecting a component of said sample that is causing said at least one peak.

### Claim 2 (depends on 1)

The method of claim 1, wherein said specific peak is the first peak on said chromatogram.

### Claim 3 (depends on 1)

The method of claim 1, wherein said specific peak is a peak at a predetermined position on said chromatogram.

### Claim 4 (depends on 1)

The method as in claim 1, and further comprising the steps of:
adding a material having a predetermined retention time to said sample prior to said collecting in order to provide said specific peak.

### Claim 5 (depends on 4)

The method as in claim 4, and further comprising the steps of:
setting a detection level for said peaks of said sample components;
providing that said specific peak has a predetermined height greater than peaks which are caused by the components of said sample and;
setting a specific detection level, higher than said detection level for said peaks of said sample components, for said specific peak so that a peak has exceeded said specific level may be identified as said specific peak.

### Claim 6 (depends on 1)

The method of claim 1, and further comprising the steps of:
providing at least one particular time band of said second group, between and adjacent two of said time bands of said first group;
entering zero for said units of time of said particular time band, so that a particular peak resulting from a single sample component can extend over said particular time band of said second group and in said two time bands of said first group; and
collecting each of two separate portions of the single sample component causing said particular peak in corresponding separate ones of said two time bands of said first group.