# US 5,350,502 A · Apparatus for fluid treatment of framed membranes

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- Number: US 5,350,502 A
- Document ID: US5350502A
- Status: Granted
- Kind: A
- Application number: 07/997,820
- Filed: 1992-12-29
- Granted: 1994-09-27
- Earliest claimed: 1992-12-29
- Assignees: E. I. Du Pont de Nemours and Company
- Inventors: Donald H. Brunk; David J. Regester; Charles W. Robertson; Anders J. Wellings
- Web page: https://patentgrep.com/patent/US5350502A

## CPC

- G01N 27/44717: {Arrangements for investigating the separated zones, e.g. localising zones}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A disclosure is made of automatic apparatus for processing transfer membranes to which a blot pattern of molecular fragments has been bound so that the pattern may be optically visualized. The apparatus is characterized by at least one open processing cell and two systems for pumping; one selectively controlled to direct fluid to and from functional units and one which is closely coupled to the cell and recirculates the fluid in the cell to assure adequate and thorough treatment therein by uniform fluid flow across both sides of the membrane.

## Claims

### Claim 1

Apparatus for the fluid treatment of a supported membrane having first and second sides and a region containing a latent blot image thereon, comprising:
(a) a processing chamber enclosing the membrane in the region containing the latent blot image, said chamber comprising an inlet port and an outlet port;
(b) means to uniformly introduce fluid into said chamber through said inlet port, in an amount sufficient to submerge the region of the membrane containing the latent blot image;
(c) means to circulate the fluid within said chamber such that the fluid flows uniformly across the first and second sides of the membrane and the region containing the latent blot image; and
(d) means to uniformly extract fluid from said chamber through said outlet port; wherein said processing chamber (a) further comprises first and second enclosure portions adapted to receive the supported membrane therebetween along at least one peripheral edge thereof, and means to translate the supported membrane into and out of said chamber (a).

### Claim 2 (depends on 1)

The apparatus of claim 1 wherein said first and second enclosure portions are joined together in a hinged relation, so that said enclosure portions provide an open position which accommodates the receiving of said supported membrane and a closed position so that said supported membrane is secured therebetween.

### Claim 3 (depends on 1)

The apparatus of claim 1 wherein said means to translate the supported membrane comprises at least one pair of opposed rollers suitably supported in relation to said processing chamber (a) and which contact the supported membrane therebetween along a peripheral edge thereof, which act in concert to displace the membrane toward or away from said processing chamber (a).

### Claim 4 (depends on 1)

The apparatus of claim 1 wherein said means to translate the supported membrane comprises one or more clips suitably supported in relation to said processing chamber (a) and which attach to the supported membrane, which act to displace the membrane toward or away from said processing chamber (a) .

### Claim 5 (depends on 1)

The apparatus of claim 1 wherein said means (b) to introduce fluid into said chamber comprises a plurality of manifolds (i) positioned so that fluid contacts the first and second sides of the membrane, and said means (d) to extract fluid comprises one or more manifolds (ii) positioned so that fluid drains from said chamber.

### Claim 6 (depends on 5)

The apparatus of claim 5 wherein said manifolds (i) are selectively connected to a plurality of fluid sources.

### Claim 7 (depends on 6)

The apparatus of claim 6 wherein said fluid sources include at least one treating-fluid.

### Claim 8 (depends on 6)

The apparatus of claim 6 wherein said fluid sources include at least one wash-fluid.

### Claim 9 (depends on 6)

The apparatus of claim 6 further including metering means (e) positioned in fluid connection with said manifolds (ii), and being adapted to transfer fluids from a plurality of fluid sources without cross contamination, and to circulate fluid in said chamber.

### Claim 10 (depends on 9)

The apparatus of claim 9 further including pumping means (f) positioned in fluid connection with said manifolds (i) and (ii) and being adapted to circulate fluids from said manifolds (i) or (ii) to said manifolds (ii) or (i), respectively.

### Claim 11 (depends on 10)

The apparatus of claim 10 operated by control means.

### Claim 12 (depends on 1)

The apparatus of claim 1 wherein said means (b) to introduce fluid into said chamber comprises at least one manifold (i) positioned so that fluid contacts the first and second sides of the membrane, and said means (d) to extract fluid comprises a pair of overflow weirs (ii) positioned so that fluid drains equally from both sides of said membrane and drains said chamber.