# US 5,349,124 A · Insect-resistant lettuce plants

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- Number: US 5,349,124 A
- Document ID: US5349124A
- Status: Granted
- Kind: A
- Application number: 07/836,149
- Filed: 1992-02-13
- Granted: 1994-09-20
- Earliest claimed: 1988-04-25
- Assignees: Monsanto Company
- Inventors: David A. Fischhoff; Stephen G. Rogers
- Web page: https://patentgrep.com/patent/US5349124A

## CPC

- C12N 15/8286: {for insect resistance}
- C07K 14/325: Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

This present invention describes genetically transformed lettuce cells and transgenic lettuce plants which exhibit toxicity to Lepidopteran larvae.

## Claims

### Claim 1

A plant of the genus Lactuca which has been regenerated from a plant cell transformed to comprise a full length Bacillus thuringiensis crystal protein gene capable of encoding a Bacillus thuringiensis crystal protein of approximately 130-135 kD under control of a promoter such that said gene is expressible in said plant in amounts insecticidal to Lepidopteran insects.

### Claim 2

A plant cell of genus Lactuca transformed to comprise a full length Bacillus thuringiensis crystal protein gene capable of encoding a Bacillus thuringiensis crystal protein of approximately 130-135 kD under control of a promoter such that said gene is expressible in said plant cell in amounts insecticidal to Lepidopteran insects.

### Claim 3 (depends on 1)

The plant of claim 1 in which the plant is Lactuca sativa.

### Claim 4 (depends on 1)

The plant of claim 1 in which the coding sequence encodes the protein encoded by the DNA sequence 1-3471 of FIG. 1.

### Claim 5 (depends on 1)

The plant of claim 1 which exhibits toxicity toward Heliothis virescens.

### Claim 6 (depends on 4)

The plant of claim 4 which exhibits toxicity toward Heliothis virescens.

### Claim 7 (depends on 2)

The cell of claim 2 in which the plant cell is Lactuca sativa.

### Claim 8 (depends on 2)

The cell of claim 2 in which the coding sequence encodes the protein encoded by the DNA sequence 1-3471 of FIG. 1.

### Claim 9 (depends on 2)

The cell of claim 2 which exhibits toxicity toward Heliothis virescens.

### Claim 10 (depends on 8)

The cell of claim 8 which exhibits toxicity toward Lepidopteran of the genus Heliothis virescens.