# US 5,349,121 A · Biologically pure mushroom culture and method for mushroom cultivation

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- Number: US 5,349,121 A
- Document ID: US5349121A
- Status: Granted
- Kind: A
- Application number: 08/089,593
- Filed: 1993-07-12
- Granted: 1994-09-20
- Earliest claimed: 1990-10-01
- Assignees: Takara Shuzo Co., Ltd.
- Inventors: Yoshio Yoshihama; Katsuhiko Kusakabe; Susumu Matsui; Hideo Morita
- Web page: https://patentgrep.com/patent/US5349121A

## CPC

- A01H 15/00: Fungi; Lichens (fungal microorganisms C12N1/14)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A biologically pure culture of Lyophyllum decastes capable of forming a fruiting body in an artificial medium. Also provided is a method for cultivation of Lyophyllum decastes to form the fruiting body.

## Claims

### Claim 1

A biologically pure culture of a strain belonging to Lyophyllum decastes capable of forming a fruiting body of about 140 grams or more per 550 grams of artificial medium containing sawdust, rice bran, and leaf mold or composted bark, for about 90 days cultivation and having all the identifying characteristics of Lyophyllum decastes, selected from the group consisting of Lyophyllum decastes K-3303 (FERM P-11320; FERM BP 4347), Lyophyllum decastes K-3304 (FERM P-11321; FERM BP4348), and Lyophyllum decastes K-3305 (FERM P-11322; FERM BP4349).

### Claim 2 (depends on 1)

A method for cultivation of Lyophyllum decastes to form a fruiting body, which comprises a step of inoculating a strain of claim 1 to an artificial medium containing sawdust and rice bran, and a cultivating step of cultivating said strain under conditions effective to form said fruiting body, which cultivation step does not include cultivating said strain while said medium is upside down.