# US 5,348,963 A · Method of screening for modulators of amyloid formation

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- Number: US 5,348,963 A
- Document ID: US5348963A
- Status: Granted
- Kind: A
- Application number: 08/053,415
- Filed: 1993-05-26
- Granted: 1994-09-20
- Earliest claimed: 1991-12-17
- Assignees: The Rockefeller University
- Inventors: Samuel E. Gandy; Gregg L. Caporaso; Paul Greengard
- Web page: https://patentgrep.com/patent/US5348963A

## CPC

- A61K 31/70: Carbohydrates; Sugars; Derivatives thereof (sorbitol A61K31/047)
- A61K 31/365: Lactones
- A61K 31/47: Quinolines; Isoquinolines
- A61P 25/28: for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Agents which modulate or affect the intracellular trafficking and processing of proteins in the mammalian cell can be utilized to affect the trafficking and processing of APP, thereby inhibiting production of Alzheimer type amyloidosis. Particularly useful agents are chloroquine and its related derivatives such as primaquine.

## Claims

### Claim 1

An in vitro assay method for screening for agents that modulate amyloid formation which comprises;
(a) metabolically labeling mammalian cells in culture to produce metabolically labeled mammalian cells;
(b) contacting the metabolically labeled mammalian cells with an agent suspected of being capable of modulating the trafficking and processing of APP in the mammalian cell during cell metabolism;
(c) lysing the mammalian cells;
(d) immunoprecipitating labeled protein of aid cells with an antibody against amyloid precursor protein; add
(e) examining the immunoprecipated APP of said cells for alterations in the trafficking and processing of APP.