# US 5,348,879 A · Cell stretching method

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- Number: US 5,348,879 A
- Document ID: US5348879A
- Status: Granted
- Kind: A
- Application number: 08/020,267
- Filed: 1993-02-19
- Granted: 1994-09-20
- Earliest claimed: 1990-08-24
- Assignees: The General Hospital Corporation; Massachusetts Institute of Technology
- Inventors: Alan R. Shapiro; Martha L. Gray; Luis A. Melendez; Jonathan L. Schaffer; Wright John D.; Jose G. Venegas
- Web page: https://patentgrep.com/patent/US5348879A

## CPC

- C12M 35/04: {Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

Disclosed is a method for imparting to a living culture of cells biaxial mechanical forces which approximate the mechanical forces to which cells are subject in vivo. The method utilizes a displacement applicator which may be actuated to contact and stretch a membrane having a living cell culture mounted thereon. Stretching of the membrane imparts biaxial mechanical forces to the cells. These forces may be uniformly applied to the cells, or they may be selectively nonuniformly applied.

## Claims

### Claim 1

A method of imparting biaxial strain to a culture of living cells, comprising the steps of:
providing one or more biocompatible membranes, each being secured about its entire periphery to provide each membrane with exposed upper and lower membrane surfaces;
disposing a cell culture having living cells upon the upper, exposed surface of each membrane;
providing an applicator means for applying a stretching force to the upper, exposed surface of each membrane, said applicator means having a membrane facing surface with a diameter slightly less than that of said exposed, lower membrane surface and having an irregular membrane-contacting surface such that raised areas of the membrane-contacting surface of the applicator means exclusively contact said exposed, lower membrane surface; and
cyclically raising and lowering said applicator means, in a predetermined cycle, to apply a biaxial strain to each membrane and to the cells within the cell culture mounted thereon, the biaxial strain being applied in a uniform or selectively non-uniform profile.

### Claim 2 (depends on 1)

The method of claim 1 wherein said biaxial strain is uniformly applied to the living cells.

### Claim 3 (depends on 1)

The method of claim 1 wherein said biaxial strain is non-uniformly applied to the living cells in selected strain profiles.