# US 5,348,871 A · Process for converting cellulosic materials into fuels and chemicals

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- Number: US 5,348,871 A
- Document ID: US5348871A
- Status: Granted
- Kind: A
- Application number: 07/884,506
- Filed: 1992-05-15
- Granted: 1994-09-20
- Earliest claimed: 1992-05-15
- Assignees: Martin Marietta Energy Systems, Inc.
- Inventors: Charles D. Scott; Brendlyn D. Faison; Brian H. Davison; Jonathan Woodward
- Web page: https://patentgrep.com/patent/US5348871A

## CPC

- C12P 19/14: produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase}
- C12N 1/205: Bacterial isolates
- C12P 1/04: by using bacteria
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- Y02E 50/10: Biofuels, e.g. bio-diesel
- Y10S 435/813: Continuous fermentation
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02E: REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A process for converting cellulosic materials, such as waste paper, into fuels and chemicals utilizing enzymatic hydrolysis of the major constituent of paper, cellulose. A waste paper slurry is contacted by cellulase in an agitated hydrolyzer. The cellulase is produced from a continuous, columnar, fluidized-bed bioreactor utilizing immobilized microorganisms. An attritor and a cellobiase reactor are coupled to the agitated hydrolyzer to improve reaction efficiency. The cellulase is recycled by an adsorption process. The resulting crude sugars are converted to dilute product in a fluidized-bed bioreactor utilizing microorganisms. The dilute product is concentrated and purified by utilizing distillation and/or a biparticle fluidized-bed bioreactor system.

## Claims

### Claim 1

A process for converting cellulosic material into glucose and ethanol comprising the steps of:
shredding a cellulosic material to increase the surface area of the cellulosic material;
mixing the shredded cellulosic material with a sufficient amount of water to form a slurry;
introducing the slurry into a first reaction vessel;
introducing cellulase into the first reaction vessel under conditions suitable to cause a hydrolysis reaction of the cellulose, the hydrolysis reaction forming glucose and cellobiose from the cellulose;
circulating the slurry from the first reaction vessel through an attritor and then through a cellobiase reactor and back to the first reaction vessel, the attritor comprising a centrifugal pump, the attritor being in fluid communication with the first reaction vessel, the cellobiase reactor comprising a fixed bed of immobilized cellobiase, the cellobiase reactor being in fluid communication with the attritor and the first reaction vessel, the attritor providing increased surface area to the cellulosic material, the cellobiase reactor providing continuous removal of cellobiose from the first reaction vessel;
recovering from the first reaction vessel a first hydrolysis product stream, the first hydrolysis product stream containing glucose, cellulase and particulate matter;
filtering the first hydrolysis product stream to remove particulate matter and to form a second hydrolysis product stream, the second hydrolysis product stream containing cellulase and glucose;
contacting a first sorbent with the second hydrolysis product stream at a basic pH to sorb the cellulase to form a cellulase-sorbent complex;
contacting the cellulase-sorbent complex with a solution having an acidic pH to separate the cellulase from the cellulase-sorbent complex;
returning the cellulase to the first reaction vessel;
fermenting the glucose of the second hydrolysis product stream to ethanol by action of a biocatalyst in a biparticle fluidized-bed bioreactor, the biparticle fluidized-bed bioreactor containing the biocatalyst and a second sorbent, the biocatalyst being Zymomonas mobilis, the ethanol being sorbed by the second sorbent to form an ethanol-sorbent complex; and
desorbing the ethanol from the ethanol-sorbent complex.

### Claim 2 (depends on 1)

The process of claim 1, wherein the cellulosic material is paper.

### Claim 3 (depends on 1)

The process of claim 1, wherein the step of circulating the slurry through the attritor further comprises the steps of:
introducing the slurry into the attritor; and
activating the attritor so that the slurry is circulated through the attritor so that the cellulosic material is attrited, thereby resulting in increased surface area of the cellulosic material where the hydrolysis reaction can occur.

### Claim 4 (depends on 1)

The process of claim 1, wherein the step of circulating the slurry through the cellobiase reactor and removing the cellobiose further comprises the steps of:
introducing the slurry into the cellobiase reactor;
reacting the cellobiose with the cellobiase of the cellobiase reactor to form glucose; and
returning the slurry and the glucose to the reaction vessel.

### Claim 5 (depends on 1)

The process of claim 1, wherein the first sorbent is contained in at least one fixed-bed enzyme sorber.

### Claim 6 (depends on 1)

The process of claim 1, wherein the biparticle fluidized-bed bioreactor comprises:
a second reaction vessel;
a fluid first phase flowing through the second reaction vessel, the fluid first phase comprising the second hydrolysis product stream;
a fluidized particulate second phase in the second reaction vessel, the second phase being retained within the second reaction vessel, the fluidized particulate phase comprising the biocatalyst; and
a particulate third phase in the second reaction vessel, the third phase migrating through the second reaction vessel, the particulate third phase comprising the second sorbent.

### Claim 7 (depends on 5)

The process of claim 5, wherein at least two fixed-bed enzyme sorbers are alternately active.

### Claim 8 (depends on 6)

The process of claim 6, wherein the second sorbent flows cocurrently with respect to the fluid first phase.

### Claim 9 (depends on 6)

The process of claim 6, wherein the second sorbent flows countercurrently with respect to the fluid first phase.