# US 5,348,866 A · Genes encoding porcine zona pellucida protein PZP-4, expression thereof and contraceptive vaccines comprising expressed (poly) peptides

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- Number: US 5,348,866 A
- Document ID: US5348866A
- Status: Granted
- Kind: A
- Application number: 07/855,411
- Filed: 1992-03-20
- Granted: 1994-09-20
- Earliest claimed: 1992-01-10
- Assignees: Shinzo Isojima; Tonen Corporation
- Inventors: Shinzo Isojima; Akiko Akatani; Yuichi Okazaki; Masanobu Sugimoto
- Web page: https://patentgrep.com/patent/US5348866A

## CPC

- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- A61K 39/00: Medicinal preparations containing antigens or antibodies (materials for immunoassay G01N33/53)
- Y10S 424/811: involving sex selection or contraception
- Y10S 530/853: Ovary; eggs; embryos
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

This invention relates to a DNA sequence comprising at least part of a porcine zona pellucida PZP-4.alpha. or -4.beta. gene coding for an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, respectively, in SEQUENCE LISTING. This invention also relates to an expression system and to expression of said DNA. In addition, this invention relates to an immunogenic recombinant polypeptide or peptide which comprises at least part of said amino acid sequence and which is obtained by expressing said DNA, and to a contraceptive vaccine for use in human or other animals which comprises said polypeptide or peptide as an active ingredient.

## Claims

### Claim 1

DNA encoding PZP-4.alpha. having SEQ ID NO: 3.

### Claim 2

DNA encoding PZP-4.beta. having SEQ ID NO: 4.

### Claim 3 (depends on 1)

An expression vector containing the DNA according to claim 1.

### Claim 4 (depends on 2)

An expression vector containing the DNA according to claim 2.

### Claim 5

Plasmid pMAL-PZP4.

### Claim 6 (depends on 3)

A host cell transformed with the expression vector according to claim 3.

### Claim 7 (depends on 4)

A host cell transformed with the expression vector according to claim 4.

### Claim 8 (depends on 5)

A host cell pMAL-PZP4/HB101 strain transformed with the plasmid pMAL-PZP4 of claim 5.

### Claim 9 (depends on 1)

A process for the production of a recombinant PZP-4.alpha. polypeptide having an amino acid sequence shown in SEQ ID NO: 1, which comprises the steps of:
(a) constructing an expression vector which contains the DNA according to claim 1,
(b) introducing said expression vector into a host cell to obtain a transformant;
(c) culturing said transformant in a suitable medium to express said DNA; and
(d) recovering and purifying the expressed polypeptide.

### Claim 10 (depends on 2)

A process for the production of a recombinant PZP-4.beta. polypeptide having an amino acid sequence shown in SEQ ID NO: 2, which comprises the steps of:
(a) constructing an expression vector which contains the DNA according to claim 2,
(b) introducing said expression vector into a host cell to obtain a transformant;
(c) culturing said transformant in a suitable medium to express said DNA; and
(d) recovering and purifying the expressed polypeptide.