# US 5,348,865 A · Genome coding phytolacca antiviral protein and a recombinant expression vector therefor

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- Number: US 5,348,865 A
- Document ID: US5348865A
- Status: Granted
- Kind: A
- Application number: 08/138,636
- Filed: 1993-10-15
- Granted: 1994-09-20
- Earliest claimed: 1993-08-28
- Assignees: Jinro Limited
- Inventors: Young-Ho Moon; Hong-Seob Jeon; Kyu-Whan Choi; Kwan-Ho Lee; Man-Keun Kim
- Web page: https://patentgrep.com/patent/US5348865A

## CPC

- C12N 15/8283: {for virus resistance}
- C07K 14/415: from plants
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome expression and a microorganism transformed therewith. PIP genome of the present invention has nucleotide hornology of about 82%, compared with the genome of Phytolacca americana antiviral protein isolated from Phytolacca americana L., which is closely related to the Phytolacca insularis Nakai. PIP cDNA is consist of 918 bp of one open reading frame and termination codon; and polyaclenylation signal which is ubiquitous in mRNA of most plants and animals, appears to be located in the upstream of 33 bp from the polyadenylation site. Recombinant PIP of the invention was proved to inhibit the growth of E. coli HB101 transformed with said expression vector.

## Claims

### Claim 1

A nucleotide sequence coding Phytolacca insularis antiviral protein isolated from Phytolacca insularis Nakai SEQ ID No. 1 presented as: ##STR1##

### Claim 2 (depends on 1)

A recombinant vector pJMC511(KCCM 10041), which contains the nucleotide sequence of claim 1.

### Claim 3 (depends on 1)

An expression vector pMS 12(KCCM 10040) for Phytolacca insularis antiviral protein, which contains the nucleotide sequence of claim 1.

### Claim 4 (depends on 3)

E. coli HB101 transformed with the expression vector of claim 3.

### Claim 5 (depends on 3)

A process for preparing Phytolacca insularis antiviral protein, which comprises the step of culturing E. coli HB101 transformed with the expression vector of claim 3.