# US 5,348,864 A · Mouse vav proto-oncogene DNA and protein sequences

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 5,348,864 A
- Document ID: US5348864A
- Status: Granted
- Kind: A
- Application number: 07/646,537
- Filed: 1991-01-25
- Granted: 1994-09-20
- Earliest claimed: 1991-01-25
- Assignees: E. R. Squibb & Sons, Inc.
- Inventors: Mariano Barbacid
- Web page: https://patentgrep.com/patent/US5348864A

## CPC

- C07K 14/82: Translation products from oncogenes
- A61P 35/00: Antineoplastic agents
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

Nucleic acid sequences, particularly DNA sequences, coding for all or part of a vav mouse proto-oncogene protein or for a modified vav mouse proto-oncogene protein, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a vav mouse proto-oncogene protein or a modified vav mouse proto-oncogene protein, and methods for producing these polypeptide molecules.

## Claims

### Claim 1

An isolated nucleic acid molecule consisting of nucleic acid sequence coding for a mouse vav proto-oncogene protein having the sequence of SEQ ID NO: 2.

### Claim 2 (depends on 1)

The nucleic acid molecule according to claim 1 which is a DNA molecule and wherein the nucleic acid sequence is a DNA sequence.

### Claim 3 (depends on 2)

The DNA molecule according to claim 2 wherein the DNA sequence has the nucleic acid sequence of SEQ. ID. NO.: 1.

### Claim 4 (depends on 3)

A DNA molecule having a DNA sequence which is fully complementary to the DNA sequence according to claim 3.

### Claim 5

An expression vector comprising a DNA sequence coding for a mouse vav proto-oncogene protein having the sequence of SEQ ID NO: 2.

### Claim 6 (depends on 5)

The expression vector according to claim 5 comprising one or more control DNA sequences capable of directing the replication and/or the expression of and operatively linked to the DNA sequence coding for a mouse vav proto-oncogene protein having the sequence of SEQ ID NO: 2.

### Claim 7 (depends on 5)

The expression vector according to claim 5 wherein the DNA sequence coding for a mouse vav proto-oncogene protein has the nucleic acid sequence of SEQ. ID. NO.: 1.

### Claim 8 (depends on 5)

The expression vector according to claim 5 designated pMB24.

### Claim 9 (depends on 5)

A prokaryotic or eukaryotic host cell containing the expression vector according to claim 5.

### Claim 10 (depends on 6, 7)

A prokaryotic or eukaryotic host cell containing the expression vector according to claims 6, 7, or 8.

### Claim 11 (depends on 9)

A method for producing a polypeptide molecule which comprises a mouse vav proto-oncogene protein having the sequence of SEQ ID NO: 2 comprising culturing a host cell according to claim 9 under conditions permitting expression of the polypeptide molecule.