# US 5,348,858 A · Monoclonal antibody specific for human interleukin-1.beta. and method for detection of biologically active human interleukin-1.beta.

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- Number: US 5,348,858 A
- Document ID: US5348858A
- Status: Granted
- Kind: A
- Application number: 07/815,875
- Filed: 1992-01-06
- Granted: 1994-09-20
- Earliest claimed: 1988-10-01
- Assignees: Otsuka Pharmaceutical Co., Ltd.
- Inventors: Setsuyoshi Uetsuki; Osamu Sato; Yasuo Nakayama; Yoshikatsu Hirai
- Web page: https://patentgrep.com/patent/US5348858A

## CPC

- G01N 33/6869: {Interleukin}
- C07K 14/545: IL-1
- C07K 16/245: {IL-1}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides a monoclonal antibody characterized in that the antibody has specific reactivity on human IL-1.beta., and a process for producing the antibody. The invention also provides a method for detection of biologically active human IL-1.beta..

## Claims

### Claim 1

Monoclonal antibody 1.beta. GOM43-4 produced by hybridoma cell line FERM BP-2565.

### Claim 2 (depends on 1)

A hybridoma cell line, FERM BP-2565, that produces the monoclonal antibody of claim 1.

### Claim 3 (depends on 1)

An immunoassay method for detecting biologically active human interleukin-1.beta. which comprises the steps of:
(a) contacting a sample suspected of containing human interleukin-1.beta. with a monoclonal antibody as defined in claim 1 in order to form an immune complex; and
(b) determining the presence of said immune complex.